Supplementary material for this article can be found on the H EPATOLOGY website ( ).

jws-hep.21559.fig1.pdf74K Supplementary Fig. 1.( a ) Epon block showing the three drill holes made by a precision driller. The exact vertical drilling direction allows for the accurate matching of the two-thousand 750 nm-thick tissue sections that were thereafter made. Orientated at these drilling holes, every tenth section was photographed ( b ), not the same tissue block as shown in a ), the anatomical structures, such as islet grafts altered acini, and non-altered liver tissue, supplying and draining vessels and the liver capsule were identified on these slides and were three-dimensionally reconstructed. In ( b ) an altered acinus is visible slightly left of the middle in the lower part. The islet graft directly borders the left drill hole. Richardson stain in ( b ). The lower edge of the panel represents 4.9 mm in ( a ), and 2.8 mm in ( b ).
jws-hep.21559.fig2.pdf39K Supplementary Fig. 2.Stereomicroscopical aspect of the same liver slice 2 weeks after islet transplantation, unstained ( a ) and after Sudan-IV staining ( b ). The pale color of the liver is the result of blood removal by perfusion fixation. In ( a ), several altered liver acini can be seen as white spots. They often show a darker area in the center, which represents the transplanted islet. If located in the section plane, the TPVs originating from the engraftment region can be seen, particularly clearly in the Sudan-stain, as they were Sudan-negative in contrast to the surrounding lipid-rich liver tissue of the corresponding altered liver acinus. Altered liver acini, having a polycyclic convex outer surface in the islet model, often appear as cloverleaf-like structures. The lower edge represents 14 mm in each panel.
jws-hep.21559.fig3.pdf46K Supplementary Fig. 3.This figure depicts different views on the two three-dimensionally reconstructed simple liver acini of Figure 8 after rotation into several directions. The liver capsule, which lied in the background and was thus not visible in Figure 8, is marked in green. In ( a ), the transplanted islet is located just below the indentation between the two acini. The TPV of the yellow acinus is moving in a 3:30 o?clock direction, in ( b ) in a 5 o?clock direction, in ( c ) towards the observer in a 2 o?clock direction, and in ( d ) towards the observer in a 12:30 o?clock direction. In ( a ), The TPV of the white acinus is moving in a 1 o?clock direction, in ( b ) in a 2:30 o?clock direction, in ( c ) into the background in a 1 o?clock direction, and in ( d ) into the background in a 2:30 o'clock direction.

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