Supplementary materials for this article can be found on the H EPATOLOGY website ( ).

jws-hep.21570.fig1.pdf18K Suppl Fig. 1. Detection of ShRNA Plasmid in vivo. Lane 1, 1KB Ladder. Lane 2, ShRNA vector control, Lane 3, Time 0, Lane 4, 12 hours post PHx. Lane 5, Day 1 post PHx. Lane 6. Day 2 post PHx. Lane 7. Day 3 post PHx. Lane 8, GAPDH, time 0, Lane 9. GAPDH 12 hours post PHx, Lane 10.GAPDH Day 1 post PHx. Lane 11, GAPDH Day 2 post PHx. Lane 12, GAPDH Day 3 post PHx, Lane 13, GAPDH, Normal non-injected control, Lane 14, normal non-injected GFP control.
jws-hep.21570.fig2A.pdf48K Suppl Fig. 2. A. Lack of off-target effects after injection of ShHGF compared to scrambled, mismatch injected, normal PHx and sham controls. Expression intensities of the top 500 expressed genes were plotted. The individual line fitted for the ShRNA treatment group sorted out separately from normal liver and sham operated animals indicating that the overall patterns of gene expression after PHx were not significantly affected by ShRNA treatment.
jws-hep.21570.fig2B.pdf48K B. Lack of induction of interferon and Interferon inducible genes by ShRNA. RNA from three rats at 24 hour post PHx was isolated and pooled. Expression values derived from the oligo-array for the different treatment groups for seven genes known to be associated with an induction of immune response were plotted. There was no significant difference in the expression of these genes among the different treatment groups and ShRNA treatment did not up-regulate expression of interferon response genes.
jws-hep.21570.mat.pdf118KSupporting Information file jws-hep.21570.mat.pdf

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