Potential conflict of interest: Nothing to report.
Cell cycle effects resulting from inhibition of hepatocyte growth factor and its receptor c-Met in regenerating rat livers by RNA interference†
Version of Record online: 10 APR 2007
Copyright © 2007 American Association for the Study of Liver Diseases
Volume 45, Issue 6, pages 1471–1477, June 2007
How to Cite
Paranjpe, S., Bowen, W. C., Bell, A. W., Nejak-Bowen, K., Luo, J.-H. and Michalopoulos, G. K. (2007), Cell cycle effects resulting from inhibition of hepatocyte growth factor and its receptor c-Met in regenerating rat livers by RNA interference. Hepatology, 45: 1471–1477. doi: 10.1002/hep.21570
- Issue online: 29 MAY 2007
- Version of Record online: 10 APR 2007
- Manuscript Accepted: 4 JAN 2007
- Manuscript Received: 11 AUG 2006
- NIH. Grant Numbers: CA35373, CA103958
- Rangos Fund for Enhancement of Research in Pathology
Supplementary materials for this article can be found on the H EPATOLOGY website ( http://interscience.wiley.com/jpages/0270-9139/suppmat/index.html ).
|jws-hep.21570.fig1.pdf||18K||Suppl Fig. 1. Detection of ShRNA Plasmid in vivo. Lane 1, 1KB Ladder. Lane 2, ShRNA vector control, Lane 3, Time 0, Lane 4, 12 hours post PHx. Lane 5, Day 1 post PHx. Lane 6. Day 2 post PHx. Lane 7. Day 3 post PHx. Lane 8, GAPDH, time 0, Lane 9. GAPDH 12 hours post PHx, Lane 10.GAPDH Day 1 post PHx. Lane 11, GAPDH Day 2 post PHx. Lane 12, GAPDH Day 3 post PHx, Lane 13, GAPDH, Normal non-injected control, Lane 14, normal non-injected GFP control.|
|jws-hep.21570.fig2A.pdf||48K||Suppl Fig. 2. A. Lack of off-target effects after injection of ShHGF compared to scrambled, mismatch injected, normal PHx and sham controls. Expression intensities of the top 500 expressed genes were plotted. The individual line fitted for the ShRNA treatment group sorted out separately from normal liver and sham operated animals indicating that the overall patterns of gene expression after PHx were not significantly affected by ShRNA treatment.|
|jws-hep.21570.fig2B.pdf||48K||B. Lack of induction of interferon and Interferon inducible genes by ShRNA. RNA from three rats at 24 hour post PHx was isolated and pooled. Expression values derived from the oligo-array for the different treatment groups for seven genes known to be associated with an induction of immune response were plotted. There was no significant difference in the expression of these genes among the different treatment groups and ShRNA treatment did not up-regulate expression of interferon response genes.|
|jws-hep.21570.mat.pdf||118K||Supporting Information file jws-hep.21570.mat.pdf|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.