Supplementary material for this article can be found on the H EPATOLOGY website ( ).

jws-hep.21571.fig1.pdf2360K Suppl Fig.1:Glucocorticoid and HNF4? regulated genes in vivo. (A) Putative HNF4? targets as revealed by microarray and genomic sequence analyses. The profiles shown are for both circadian and non-circadian transcripts induced by dexamethasone administration at CT6 (DEX). Inductions at 5 h (CT11), 17 h (CT23) and 29 h (CT35) post-injection are shown for both sets of targets. Only genes that contain HNF4? regulatory sequences, but not GREs, within their 5?-flanking and/or intronic DNA are shown; these represent likely HNF4? targetsin vivo . (B) Detailed, profiles illustrating the effects of VEH or DEX on the transcription of the circadian and HNF4?-regulated genes Bnip3, Nfil3, Pklr and Ahcy. Mean + s.e.m. (n=3) * p < 0.05. (C-D) Hepatic expression of HNF4? target genes (C) Fgf18, (D) Ddit3 and (E) Jam3 showing high-resolution circadian profiles, acute induction by DEX in SCN ablated mice, and the effect of HNF4? null mutation on gene expression.All high-resolution circadian profiles are based on the mean ± s.e.m. of three independent real-time PCR assays using pooled RNA from n=6 animals. Data from SCNX mice treated with VEH or DEX are mean ±s.e.m n= 3 mice. For HNF4? mutant mice, each value is the mean ±s.e.m. for n=6 animals in each group. * p < 0.05.
jws-hep.21571.tbl1.pdf5646K Suppl Table 1:Analysis of glucocorticoid-induced circadian genes and showing the presence of glucocorticoid response elements (GREs), HNF4alpha sequences, E/E?-boxes, D-boxes and ROREs in 5? and intronic regulatory sequence (see main text for details).

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