fax: (44) 1223-402310
Glucocorticoid signaling synchronizes the liver circadian transcriptome†
Version of Record online: 30 MAY 2007
Copyright © 2007 American Association for the Study of Liver Diseases
Volume 45, Issue 6, pages 1478–1488, June 2007
How to Cite
Reddy, A. B., Maywood, E. S., Karp, N. A., King, V. M., Inoue, Y., Gonzalez, F. J., Lilley, K. S., Kyriacou, C. P. and Hastings, M. H. (2007), Glucocorticoid signaling synchronizes the liver circadian transcriptome. Hepatology, 45: 1478–1488. doi: 10.1002/hep.21571
Potential conflict of interest: Nothing to report.
- Issue online: 30 MAY 2007
- Version of Record online: 30 MAY 2007
- Manuscript Accepted: 5 JAN 2007
- Manuscript Received: 31 JUL 2006
- Guggenheim Foundation and a Royal Society Wolfson Research Merit Award
- St. John's College, Cambridge
Supplementary material for this article can be found on the H EPATOLOGY website ( http://interscience.wiley.com/jpages/0270-9139/suppmat/index.html ).
|jws-hep.21571.fig1.pdf||2360K||Suppl Fig.1:Glucocorticoid and HNF4? regulated genes in vivo. (A) Putative HNF4? targets as revealed by microarray and genomic sequence analyses. The profiles shown are for both circadian and non-circadian transcripts induced by dexamethasone administration at CT6 (DEX). Inductions at 5 h (CT11), 17 h (CT23) and 29 h (CT35) post-injection are shown for both sets of targets. Only genes that contain HNF4? regulatory sequences, but not GREs, within their 5?-flanking and/or intronic DNA are shown; these represent likely HNF4? targetsin vivo . (B) Detailed, profiles illustrating the effects of VEH or DEX on the transcription of the circadian and HNF4?-regulated genes Bnip3, Nfil3, Pklr and Ahcy. Mean + s.e.m. (n=3) * p < 0.05. (C-D) Hepatic expression of HNF4? target genes (C) Fgf18, (D) Ddit3 and (E) Jam3 showing high-resolution circadian profiles, acute induction by DEX in SCN ablated mice, and the effect of HNF4? null mutation on gene expression.All high-resolution circadian profiles are based on the mean ± s.e.m. of three independent real-time PCR assays using pooled RNA from n=6 animals. Data from SCNX mice treated with VEH or DEX are mean ±s.e.m n= 3 mice. For HNF4? mutant mice, each value is the mean ±s.e.m. for n=6 animals in each group. * p < 0.05.|
|jws-hep.21571.tbl1.pdf||5646K||Suppl Table 1:Analysis of glucocorticoid-induced circadian genes and showing the presence of glucocorticoid response elements (GREs), HNF4alpha sequences, E/E?-boxes, D-boxes and ROREs in 5? and intronic regulatory sequence (see main text for details).|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.