Engulfment of apoptotic cells expressing HCV proteins leads to differential chemokine expression and STAT signaling in human dendritic cells†
Version of Record online: 30 MAY 2007
Copyright © 2007 American Association for the Study of Liver Diseases
Volume 45, Issue 6, pages 1422–1432, June 2007
How to Cite
Wertheimer, A. M., Polyak, S. J., Leistikow, R. and Rosen, H. R. (2007), Engulfment of apoptotic cells expressing HCV proteins leads to differential chemokine expression and STAT signaling in human dendritic cells. Hepatology, 45: 1422–1432. doi: 10.1002/hep.21637
Potential conflict of interest: Nothing to report.
- Issue online: 30 MAY 2007
- Version of Record online: 30 MAY 2007
- Manuscript Accepted: 8 JAN 2007
- Manuscript Received: 22 FEB 2006
- National Institutes of Health grants. Grant Numbers: DK62187GCRC, RO1 DK060590
- American Liver Foundation Liver Scholar award
- Portland Veterans Administration Research Enhancement Award Program, HCV Center Grant. Grant Number: U19 A 1066328
- Public Health Services Grant. Grant Number: 5 M01 RR000334
- OHSU General Clinical Research Center
Supplementary material for this article can be found on the H EPATOLOGY website ( http://interscience.wiley.com/jpages/0270-9139/suppmat/index.html ).
|jws-hep.21637.fig1.tif||1544K||Figure S1. Immunoblot demonstrating expression of HCV core-E1 (20 kD), and NS5a (56 kD) proteins after LCL infection with HCV-vaccinia recombinant viruses.Antigen lanes include uninfected cells (B cell lymphoid cell line (LCL)), cells infected with wild type vaccinia virus (vWT), and cells infected with various HCV-vaccinia constructs (rVV1, rVV3, rVV6 (see Figure 1 for map). Anti-HCV poly- and monoclonal antibodies, core (a gift from Chiron), and NS5a (Virostat) respectively were used to visualize the specific HCV bands at the expected locations.|
|jws-hep.21637.fig2.tif||29158K||Figure S2: Generation of monocyte derived DCs and characterization of non-adherent cells.Human peripheral blood cells were adhered to plastic, and cultured for 5 days to generate DCs. Flow cytometry analysis was performed on the non-adherent cells as well as the 5 day cultured cells. Phenotypic cell surface stains as illustrated on the X and Y axis.|
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