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Identification of PFTAIRE protein kinase 1, a novel cell division cycle-2 related gene, in the motile phenotype of hepatocellular carcinoma cells†
Article first published online: 8 JUN 2007
Copyright © 2007 American Association for the Study of Liver Diseases
Volume 46, Issue 2, pages 436–445, August 2007
How to Cite
Pang, E. Y.-T., Bai, A. H.-C., To, K.-F., Sy, S. M.-H., Wong, N. L.-Y., Lai, P. B.-S., Squire, J. A. and Wong, N. (2007), Identification of PFTAIRE protein kinase 1, a novel cell division cycle-2 related gene, in the motile phenotype of hepatocellular carcinoma cells. Hepatology, 46: 436–445. doi: 10.1002/hep.21691
Potential conflict of interest: Nothing to report.
- Issue published online: 27 JUL 2007
- Article first published online: 8 JUN 2007
- Manuscript Accepted: 29 JAN 2007
- Manuscript Received: 1 JUL 2006
- Hong Kong Research Grants Council. Grant Numbers: CUHK 4467/03M, CUHK 4418/05M, CUHK2/02C
- Strategic Research
- Li Ka-Shing Institute of Health, The Chinese University of Hong Kong
Supplementary material for this article can be found on the H EPATOLOGY website ( http://interscience.wiley.com/jpages/0270-9139/suppmat/index.html ).
|jws-hep.21691.tif||318K||Supplementary Figure I. Stable knockdown of PFTK1 in Hep3B by vector-based short hairpin RNA (shRNA) interference (A)Stable knockdown of PFTK1 in 3 clones of pGE1-shPFTK1 cells did not present an effect on cell viability compared to control cells (p>0.6).(B)In wound-healing assay, cells in control experiment showed outgrowth into the open wound after 24h, whereas stably transfected cells showed considerable retardation in cell motility.(C)A significant reduction in the invasive property and chemotactic cell migration was also observed in pGE1-shPFTK1 transfected Hep3B cells (***p<0.0005; **p<0.001; *p<0.05).|
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