Comparison of hepatic properties and transplantation of Thy-1+ and Thy-1− cells isolated from embryonic day 14 rat fetal liver†
Article first published online: 23 JUL 2007
Copyright © 2007 American Association for the Study of Liver Diseases
Volume 46, Issue 4, pages 1236–1245, October 2007
How to Cite
Oertel, M., Menthena, A., Chen, Y.-Q. and Shafritz, D. A. (2007), Comparison of hepatic properties and transplantation of Thy-1+ and Thy-1− cells isolated from embryonic day 14 rat fetal liver. Hepatology, 46: 1236–1245. doi: 10.1002/hep.21775
Potential conflict of interest: Nothing to report.
- Issue published online: 25 SEP 2007
- Article first published online: 23 JUL 2007
- Manuscript Accepted: 11 APR 2007
- Manuscript Received: 8 JAN 2007
- National Institutes of Health. Grant Numbers: RO1-DK17609, P30-DK41296
Supplementary material for this article can be found on the H EPATOLOGY Web site ( http://interscience.wiley.com/jpages/0270-9139/suppmat/index.html ).
|jws-hep.21775.tbl1.doc||35K||Supplementary Table 1:Primer Sequences used for RT-PCR|
|jws-hep.21775.fig1.tif||6208K||Supplementary Fig. 1:Albumin and E-cadherin expression in the Thy-1 + and Thy-1- fractions after selection with anti-Thy-1 magnetic microbeads. Immunohistochemical analysis for albumin showed lower albumin expression (red) in Thy-1 + cell fractions ( A & C ) compared to the Thy-1- fetal liver cell fraction ( B & D ). Immunohistochemical analysis for E-cadherin expression (green) showed similar results, as only rare E-cadherin positive cells were detected within the Thy-1 + fetal liver cell fraction ( E ), whereas many E-cadherin positive cells were found in the Thy-1- fraction ( F ). DAPI was used for nuclei staining (blue). Data are shown as merged images. Original magnification x200 ( A, B ) and x900 ( C-F ).|
|jws-hep.217751.doc||49K||Supporting Information file jws-hep.217751.doc|
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