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jws-hep.21779.fig1.pdf40KSuppl Fig 1: OT-I CD8 T-cells were purified from the lymph nodes of OT-I mice. 4 million cells were CFSE-labelled and transferred intravenously into ASBT-OVA or TF-OVA mice. Experiments in ASBT-OVA and TF-OVA mice were carried out simultaneously and mice received cells from the same batch of OT-I T-cells. 20 hours after transfer, cells were isolated form the livers and stained for CD8 and Va2. In (A), expression levels of CD8 and Va2 within the population of CFSE-positive (transgenic) cells are depicted. In (B), numbers of CD8+Va2+ and CFSE+ cells isolated from the livers of ASBT-OVA and TF-OVA mice are compared (n=6 each).
jws-hep.21779.fig2.pdf34KSuppl Fig 2: OT-I CD8 T-cells were purified from the lymph nodes of OT-I or OT-I RAG-/- mice. Cells isolated from OT-I mice were also selected for CD62L expression. 4 million cells were CFSE-labelled and transferred intravenously into TF-OVA (A) or ASBT-OVA mice (B). T-cells were isolated from the indicated tissues (Ing. LN. Inguinal lymph node; Liv. LN. Liver lymph node; Intrahep. Intrahepatic) at 44 hours and analyzed for the presence of proliferating transgenic T-cells by flow cytometry. Plots display data gated on CD8+Va2+ cells.

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