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Autoimmune, Cholestatic, and Biliary Disease
Differential priming of CD8 and CD4 T-cells in animal models of autoimmune hepatitis and cholangitis†
Version of Record online: 26 JUL 2007
Copyright © 2007 American Association for the Study of Liver Diseases
Volume 46, Issue 4, pages 1155–1165, October 2007
How to Cite
Derkow, K., Loddenkemper, C., Mintern, J., Kruse, N., Klugewitz, K., Berg, T., Wiedenmann, B., Ploegh, H. L. and Schott, E. (2007), Differential priming of CD8 and CD4 T-cells in animal models of autoimmune hepatitis and cholangitis. Hepatology, 46: 1155–1165. doi: 10.1002/hep.21796
Potential conflict of interest: Nothing to report.
- Issue online: 25 SEP 2007
- Version of Record online: 26 JUL 2007
- Manuscript Accepted: 26 APR 2007
- Manuscript Received: 27 JAN 2007
- Deutsche Forschungsgemeinschaft. Grant Numbers: Scho776/4-2, Z1SFB633, A8SFB633
- CJ Martin Fellowship of the National Heath and Medical Research Council, Australia
Supplementary material for this article can be found on the H EPATOLOGY Web site ( http://interscience.wiley.com/jpages/0270-9139/suppmat/index.html ).
|SupplFig1.pdf||40K||Suppl Fig 1: OT-I CD8 T-cells were purified from the lymph nodes of OT-I or OT-I RAG-/- mice. Cells isolated from OT-I mice were also selected for CD62L expression. 4 million cells were CFSE-labelled and transferred intravenously into TF-OVA (A) or ASBT-OVA mice (B). T-cells were isolated from the indicated tissues (Ing. LN. Inguinal lymph node; Liv. LN. Liver lymph node; Intrahep. Intrahepatic) at 44 hours and analyzed for the presence of proliferating transgenic T-cells by flow cytometry. Plots display data gated on CD8 + Vα2 + cells.|
|SupplFig2.pdf||34K||Suppl Fig. 2: OT-I CD8 T-cells were purified from the lymph nodes of OT-I mice. 4 million cells were CFSE-labelled and transferred intravenously into ASBT-OVA or TF-OVA mice. Experiments in ASBT-OVA and TF-OVA mice were carried out simultaneously and mice received cells from the same batch of OT-I T-cells. 20 hours after transfer, cells were isolated form the livers and stained for CD8 and Vα2. In (A), expression levels of CD8 and Vα2 within the population of CFSE-positive (transgenic) cells are depicted. In (B), numbers of CD8 + Vα2 + and CFSE + cells isolated from the livers of ASBT-OVA and TF-OVA mice are compared (n=6 each).|
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