ADAM metallopeptidase with thrombospondin type 1 motif 2 inactivation reduces the extent and stability of carbon tetrachloride–induced hepatic fibrosis in mice

Authors

  • Frédéric Kesteloot,

    1. Laboratory of Connective Tissues Biology, Interdisciplinary Cluster for Applied Genoproteomics/Center for Experimental Cancer Research, University of Liège, Liège, Belgium
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  • Alexis Desmoulière,

    1. Groupe de Recherches pour l'Etude du Foie, Institut National de la Santé et de la Recherche Médicale E0362, Université Victor Segalen Bordeaux 2, Bordeaux, France
    2. Department of Physiology, Faculty of Pharmacy, University of Limoges, Limoges, France
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  • Isabelle Leclercq,

    1. Gastroenterology Unit, Université Catholique de Louvain, Brussels, Belgium
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  • Marc Thiry,

    1. Laboratoire de Biologie Cellulaire et Tissulaire, Université de Liège, Liège, Belgium
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  • Jorge E. Arrese,

    1. Department of Dermatopathology, University Hospital of Liège, Sart Tilman, Belgium
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  • Darwin J. Prockop,

    1. Center for Gene Therapy, Tulane University Health Science Center, New Orleans, LA
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  • Charles M. Lapière,

    1. Laboratory of Connective Tissues Biology, Interdisciplinary Cluster for Applied Genoproteomics/Center for Experimental Cancer Research, University of Liège, Liège, Belgium
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  • Betty V. Nusgens,

    1. Laboratory of Connective Tissues Biology, Interdisciplinary Cluster for Applied Genoproteomics/Center for Experimental Cancer Research, University of Liège, Liège, Belgium
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  • Alain Colige

    Corresponding author
    1. Laboratory of Connective Tissues Biology, Interdisciplinary Cluster for Applied Genoproteomics/Center for Experimental Cancer Research, University of Liège, Liège, Belgium
    • Laboratory of Connective Tissues Biology, Tour de Pathologie, B23/3, 4000 Sart Tilman, Belgium===

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    • fax: 32-4-366-2457


  • Potential conflict of interest: Dr. Prockop owns stock in FibroGen.

Abstract

ADAMTS2 belongs to the “ADAM metallopeptidase with thrombospondin type 1 motif” (ADAMTS) family. Its primary function is to process collagen type I, II, III, and V precursors into mature molecules by excising the aminopropeptide. This process allows the correct assembly of collagen molecules into fibrils and fibers, which confers to connective tissues their architectural structure and mechanical resistance. To evaluate the impact of ADAMTS2 on the pathological accumulation of extracellular matrix proteins, mainly type I and III collagens, we evaluated carbon tetrachloride–induced liver fibrosis in ADAMTS2-deficient (TS2−/−) and wild-type (WT) mice. A single carbon tetrachloride injection caused a similar acute liver injury in deficient and WT mice. A chronic treatment induced collagen deposition in fibrous septa that were made of thinner and irregular fibers in TS2−/− mice. The rate of collagen deposition was slower in TS2−/− mice, and at an equivalent degree of fibrosis, the resorption of fibrous septa was slightly faster. Most of the genes involved in the development and reversion of the fibrosis were similarly regulated in TS2−/− and WT mice. Conclusion: These data indicate that the extent of fibrosis is reduced in TS2−/− mice in comparison with their WT littermates. Inhibiting the maturation of fibrillar collagens may be a beneficial therapeutic approach to interfering with the development of fibrotic lesions. (HEPATOLOGY 2007.)

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