Increased susceptibility to liver injury in hepatitis B virus transgenic mice involves NKG2D-ligand interaction and natural killer cells†
Version of Record online: 11 JUL 2007
Copyright © 2007 American Association for the Study of Liver Diseases
Volume 46, Issue 3, pages 706–715, September 2007
How to Cite
Chen, Y., Wei, H., Sun, R., Dong, Z., Zhang, J. and Tian, Z. (2007), Increased susceptibility to liver injury in hepatitis B virus transgenic mice involves NKG2D-ligand interaction and natural killer cells. Hepatology, 46: 706–715. doi: 10.1002/hep.21872
Potential conflict of interest: Nothing to report.
- Issue online: 24 AUG 2007
- Version of Record online: 11 JUL 2007
- Manuscript Accepted: 13 JUN 2007
- Manuscript Received: 16 MAR 2007
- Natural Science Foundation of China. Grant Numbers: 30528007, 30570819, 30571695, 30500467, 30474585
- Ministry of Science and Technology of China. Grant Number: 973 Basic Science Project #2003CB515501
- Ministry of Education of the People's Republic of China. Grant Number: #705029
Supplementary material for this article can be found on the H EPATOLOGY Web site ( http://interscience.wiley.com/jpages/0270-9139/suppmat/index.html ).
|jws-hep.21872.fig1.pdf||119K||Supplementary Fig. 1. Cytokine production of intrahepatic NKT cells in HBs-Tg mice after a low dose of Con A injection.After 1 h stimulation of Con A (3 μg/g body weight) or PBS, intrahepatic NKT cells were analyzed by flow cytometry for the production of IFN-γ and IL-4. These were from a single experiment representative of three independent experiments. PBS, phosphatebuffered saline; Con A, concanavalin A; IFN-γ, interferon-gamma; IL-4, interleukin-4.|
|jws-hep.21872.fig2.pdf||98K||Supplementary Fig. 2. Neutralization of IFN-g or IL-4 alleviated over-sensitive liver injury in HBs-Tg mice after a low dose of Con A injection.IFN-γ(A) or IL-4 (B) was neutralized by the indicated antibody in HBs-Tg mice before Con A (3 μg/g body weight) injection respectively. Serum ALT and AST were determined 18 h after Con A injection. Data were shown as mean ± SEM from four mice in each group. Con A, concanavalin A; ALT, alanine aminotransferase; AST, aspartate aminotransferase; Ig, immunoglobulin; Ab, antibody; IFN-γ, interferon-gamma; IL-4, interleukin-4.|
|jws-hep.21872.fig3.pdf||115K||Supplementary Fig. 3. Neutralization of IFN-g or IL-4 inhibited NK cell activation in HBs-Tg mice after a low dose of Con A injection.IFN-γ or IL-4 was neutralized by the indicated antibody in HBs-Tg mice before Con A (3 μg/g body weight) injection respectively. At 18 h of Con A injection, CD69 expression on intrahepatic NK cells was analyzed by flow cytometry. The gray histogram was for the NK cells from control HBs-Tg mice with PBS injection. Ig, immunoglobulin; Ab, antibody; IFN-γ, interferon-gamma; IL-4, interleukin-4.|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.