SEARCH

SEARCH BY CITATION

In the article entitled “Lentivirus-mediated RNA interference targeting enhancer of zeste homolog 2 inhibits hepatocellular carcinoma growth through down-regulation of stathmin” (HEPATOLOGY 2007:200-2008; DOI: 10.1002/hep.21668), only panels A and B are listed in Figure 2. Below is Figure 2 in its entirety:

thumbnail image

Figure 2. Efficient suppression of EZH2 by lentiviral shRNA (Lenti-shRNA) in HCC cells. (A) Schematic diagram of Lenti-shRNA vector pLUNIG, in which shRNA was driven by a U6 promoter, and Neores and EGFP were biocistronically expressed under the control of a CMV promoter. (B) Efficient transduction of Bel7404 cells with Lenti-shRNA. Fluorescence photomicrographs of Bel7404 cells infected by Lenti-shRNA at an multiplicity of infection of 10. Pictures were taken 72 hours after infection at a magnification of ×100, scale bar = 10 μm. (C,D) Quantitative RT-PCR showing efficient knockdown of EZH2 mRNA and reduction of EZH2 protein revealed by western blot in Bel7404 cells. (E,F) Effective down-regulation of EZH2 mRNA and suppression of EZH2 protein in HepG2 cells.

Download figure to PowerPoint

The Publisher regrets the error.