Corrections


In the article “A systems biology approach to the pathogenesis of obesity-related nonalcoholic fatty liver disease using reverse phase protein microarrays for multiplexed cell signaling analysis” by Younossi et al (Hepatology 2007;46:166-172; doi: 10.1002/hep.21688), Tables 1 and 2 are missing from the printed article. Below in their entirety are Tables 1 and 2.

Table 1. Clinical and Laboratory Data for Different Groups of Patients with NAFLD
 NASH (N = 15)Steatosis with NSI (N=46)Simple Steatosis (N = 9)Controls (N = 29)
Age, years (mean ± SD)43.43 ± 12.8643.62 ± 11.3049.27 ± 13.2642.98 ± 9.20
Female, %40.00%76.09%88.89%79.31%
Race, Caucasian %93.33%73.91%88.89%72.41%
Body mass index46.65 ± 7.6146.87 ± 7.4444.18 ± 7.7044.81 ± 7.63
Waist, cm137.79 ± 16.40134.85 ± 17.64127.99 ± 12.77135.66 ± 15.59
AST level, IU/L (mean ± SD)46.14 ± 34.1923.61 ± 10.7928.33 ± 20.5419.52 ± 4.63
ALT level, IU/L (mean ± SD)58.36 ± 33.3430.98 ± 18.6039.78 ± 27.9421.96 ± 7.53
AST/ALT ratio (mean ± SD)0.79 ± 0.200.89 ± 0.410.78 ± 0.220.96 ± 0.32
Fasting serum triglyceride,mg/dL (mean ± SD)206.93 ± 103.19189.45 ± 120.88176.43 ± 90.08132.75 ± 75.50
Fasting serum cholesterol, mg/dL (mean ± SD)191.86 ± 32.02212.49 ± 53.19200.13 ± 43.95181.65 ± 29.80
Fasting serum glucose, mg/dL (mean ± SD)120.04 ± 29.82119.81 ± 48.61127.03 ± 50.71112.32 ± 31.72
Fasting serum insulin (mean ± SD)14.07 ± 7.789.44 ± 5.9211.54 ± 7.0111.54 ± 15.62
HOMA (mean ± SD)4.06 ± 2.072.78 ± 2.092.90 ± 2.043.81 ± 7.44
Table 2. Signaling Endpoints Analyzed
AntibodyCompanyDilution
Cleaved Caspase-3 (D175)Cell Signaling1:50
Cleaved Caspase-7 (D198)Cell Signaling1:100
Cleaved Caspase-9 (D315)Cell Signaling1:50
Cleaved Caspase-9 (D330)Cell Signaling1:50
COX2Upstate1:1000
IRS-1Cell Signaling1:200
p70 S6 KinaseCell Signaling1:100
Phospho-4E-BP1 (S65)Cell Signaling1:50
Phospho-AKT (S473)Cell Signaling1:100
Phospho-AKT (T308)Cell Signaling1:100
Phospho-AMPKalpha1 (S485)Cell Signaling1:100
Phospho-ASK1 (Ser83)Cell Signaling1:50
Phospho-BAD (S112)Cell Signaling1:400
Phospho-BAD (S136)Cell Signaling1:50
Phospho-BAD (S155)Cell Signaling1:200
Phospho-c-Abl (Thr735)Cell Signaling1:50
Phospho-c-Raf (S338)Cell Signaling1:200
Phospho-CREB (S133)Cell Signaling1:400
Phospho-EGFR (Y1068)Cell Signaling1:100
Phospho-eIF4G (S1108)Cell Signaling1:2000
Phospho-eNOS (S1177)Cell Signaling1:200
Phospho-eNOS/NOS III (S116)Upstate1:500
Phospho-ErbB2 (Y1248)Upstate1:1000
Phospho-ERK (T202/Y204)Cell Signaling1:2000
Phospho-Estrogen Rec a (S118)Cell Signaling1:1000
Phospho-FADD (S194)Cell Signaling1:200
Phospho-FAK (Y397)BD1:50
Phospho-FKHR (S256)Cell Signaling1:100
Phospho-FKHR (T24)/FKHRL1 (T32)Cell Signaling1:500
Phospho-GSK-3a/B (S21/9)Cell Signaling1:100
Phospho-GSK3a/B (Y279/216)BioSource1:600
Phospho-IkappaB-alpha (S32)Cell Signaling1:100
Phospho-IRS-1 (S612)Cell Signaling1:200
Phospho-MARCKs (S152/156)Cell Signaling1:200
Phospho-MEK1/2 (S217/221)Cell Signaling1:200
Phospho-mTOR (S2448)Cell Signaling1:200
Phospho-p38 MAPK (T180/Y182)Cell Signaling1:100
Phospho-p70 S6 Kinase (T389)Cell Signaling1:100
Phospho-p90RSK (S380)Cell Signaling1:400
Phospho-PKA C (T197)Cell Signaling1:1000
Phospho-PKCa/B II (T638/641)Cell Signaling1:200
Phospho-PKCdelta (T505)Cell Signaling1:100
Phospho-PTEN (S380)Cell Signaling1:500
Phospho-Pyk2 (Y402)Cell Signaling1:200
Phospho-Ras-GRF1 (S916)Cell Signaling1:50
Phospho-SAPK/JNK (T183/Y185)Cell Signaling1:200
Phospho-Shc (Y317)Upstate1:200
Phospho-SMAD2 (S465/467)Cell Signaling1:250
Phospho-Src (Y527)Cell Signaling1:500
Phospho-Stat1 (Y701)Upstate1:500
Phospho-Stat3 (S727)Cell Signaling1:100
Phospho-Stat5 (Y694)Cell Signaling1:50
Phospho-AMPKbeta1 (S108)Cell Signaling1:100
PI3-KinaseBD1:100

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