Liver Biology and Pathobiology

You have full text access to this OnlineOpen article

Characterization of ionotrophic purinergic receptors in hepatocytes†

  1. Daniel S. Emmett1,
  2. Andrew Feranchak1,
  3. Gordan Kilic1,
  4. Livia Puljak1,
  5. Bonnie Miller1,
  6. Svjetlana Dolovcak1,
  7. Ryan McWilliams2,
  8. R. Brian Doctor2,
  9. J. Gregory Fitz1,‡,*

Article first published online: 20 NOV 2007

DOI: 10.1002/hep.22035

Issue

Hepatology

Hepatology

Volume 47, Issue 2, pages 698–705, February 2008

Additional Information

How to Cite

Emmett, D. S., Feranchak, A., Kilic, G., Puljak, L., Miller, B., Dolovcak, S., McWilliams, R., Doctor, R. B. and Fitz, J. G. (2008), Characterization of ionotrophic purinergic receptors in hepatocytes. Hepatology, 47: 698–705. doi: 10.1002/hep.22035

Author Information

  1. 1

    University of Texas Southwestern Medical Center, Dallas, TX

  2. 2

    University of Colorado Health Sciences Center, Denver, CO

  1. ‡

    fax: 214-648-3088

*Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9030

  1. †

    Potential conflict of interest: Nothing to report.

Publication History

  1. Issue published online: 26 JAN 2008
  2. Article first published online: 20 NOV 2007
  3. Manuscript Accepted: 4 SEP 2007
  4. Manuscript Received: 23 APR 2007

Funded by

  • Cystic Fibrosis Foundation
  • National Institutes of Health. Grant Numbers: DK 61480, DK 43278, DK 46082

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (317K)

References

  • 1
    Wang Y, Roman RM, Lidofsky SD, Fitz JG. Autocrine signaling through ATP release represents a novel mechanism for cell volume regulation. Proc Nat Acad Sci U S A 1996; 93: 12020–12025.
  • 2
    Dranoff JA, Masyuk AI, Kruglov EA, LaRusso NF, Nathanson MH. Polarized expression and function of P2Y ATP receptors in rat bile duct epithelia. Am J Physiol Gastrointest Liver Physiol 2001; 281: G1059–G1067.
  • 3
    Roman RM, Fitz JG. Emerging roles of purinergic signaling in gastrointestinal epithelial secretion and hepatobiliary function. Gastroenterology 1999; 116: 964–979.
  • 4
    Haussinger D, Stehle T, Gerok W. Actions of extracellular ATP and UTP in perfused rat liver. Eur J Biochem 1987; 167: 65–71.
    Direct Link:
  • 5
    Feranchak AP, Roman RM, Schwiebert EM, Fitz JG. Phosphatidyl inositol 3-kinase represents a novel signal regulating cell volume through effects on ATP release. J Biol Chem 1998; 273: 14906–14911.
  • 6
    Vanstapel F, Waebens M, Van Hecke P, Decanniere C, Stalmans W. Modulation of maximal glycogenolysis in perfused rat liver by adenosine and ATP. Biochem J 1991; 277: 597–602.
  • 7
    Haussinger D, Busshardt E, Stehle T, Stoll B, Wettstein M, Gerok W. Stimulation of thromboxane release by extracellular UTP and ATP from perfused liver. Eur J Biochem 1988; 178: 249–256.
    Direct Link:
  • 8
    Keppens S, Vandekerchove A, Moshage H, Yap SH, Aerts R, De Wulf H. Regulation of glycogen phosphorylase activity in isolated human hepatocytes. HEPATOLOGY 1993; 17: 610–614.
    Direct Link:
  • 9
    Okajima F, Tokomitsu Y, Kondo Y, Ui M. P2-purinergic receptors are coupled to two signal transduction systems leading to inhibition of cAMP generation and to production of inositol trisphosphate in rat hepatocytes. J Biol Chem 1987; 262: 13483–13490.
  • 10
    North RA. Molecular physiology of P2X receptors. Physiol Rev 2002; 82: 1013–1067.
  • 11
    Soto F, Garcia-Guzman M, Gomez-Hernandez JM, Hollmann M, Karschin C, Stuhmer W. P2X4: an ATP-activated ionotrophic receptor cloned from rat brain. Proc Nat Acad Sci U S A 1996; 93: 3684–3688.
  • 12
    Doctor RB, Matzakos T, McWilliams R, Johnson S, Feranchak AP, Fitz JG. Purinergic regulation of cholangiocyte secretion: identification of a novel role for P2X receptors. Am J Physiol 2005; 288: G779–G786.
  • 13
    Fitz JG, Sostman A, Middleton JP. Regulation of cation channels in liver cells by intracellular calcium and protein kinase C. Am J Physiol 1994; 266: G677–G684.
  • 14
    Lidofsky SD, Sostman A, Fitz JG. Regulation of cation-selective channels in liver cells. J Membr Biol 1997; 157: 231–236.
  • 15
    Schlenker T, Feranchak AP, Schwake L, Stremmel W, Fitz JG. Functional interactions between oxidative stress, membrane Na+ permeability, and cell volume in hepatoma cells. Gastroenterology 2000; 118: 395–403.
  • 16
    Bodily K, Wang Y, Roman RM, Sostman A, Fitz JG. Characterization of a swelling-activated anion conductance in HTC hepatoma cells. HEPATOLOGY 1997; 25: 403–410.
    Direct Link:
  • 17
    Taylor AL, Schwiebert LM, Smith JJ, King C, Jones JR, Sorscher EJ, et al. Epithelial P2x purinergic receptor channel expression and function. J Clin Invest 1999; 104: 875–884.
  • 18
    Zsembery A, Boyce AT, Liang L, Peti-Peterdi J, Bell PD, Schwiebert EM. Sustained calcium entry through P2X nucleotide receptor channels in human airway epithelial cells. J Biol Chem 2003; 278: 133398–133408.
  • 19
    He ML, Gonzalez-Iglesias AE, Stojilkovic SS. Role of nucleotide P2 receptors in calcium signaling and prolactin release in pituitary lactotrophs. J Biol Chem 2003; 278: 46270–46277.
  • 20
    Acuna-Castillo C, Morales B, Huidobro-Toro JP. Zinc and copper modulate differentially the P2X(4) receptor. J Neurochem 2000; 74: 1529–1537.
    Direct Link:
  • 21
    Priel A, Silberberg SD. Mechanism of ivermectin facilitation of human P2X4 receptor channels. J Gen Physiol 2004; 123: 281–293.
  • 22
    Virginoi C, Church D, North RA, Surprenant A. Effects of divalent cations, protons and calmidazolium at the rat P2X7 receptor. Neuropharmacology 1997; 36: 1285–1294.
  • 23
    Acuna-Castillo C, Coddou C, Bull P, Brito J, Huidobro-Toro JP. Differential role of extracellular histidines in copper, zinc, magnesium and proton modulation of the P2X7 purinergic receptor. J Neurochem 2007; 101: 17–26.
    Direct Link:
  • 24
    Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 162: 156–159.
  • 25
    Luo, X, Zheng W, Yan M, Lee MG, Muallem S. Multiple functional P2X and P2Y receptors in the luminal and basolateral membranes of pancreatic duct cells. Am J Physiol Cell Physiol 1999; 277: C205–C215.
  • 26
    Nguyen K, Miller BC. CD28 costimulation induces delta opioid receptor expression during anti-CD3 activation of T cells. J Immunol 2002; 168: 4440–4445.
  • 27
    Dunn SD. Effects of the modification of transfer buffer composition and the renaturation of proteins in gels on the recognition of proteins on western blots by monoclonal-antibodies. Anal Biochem 1986; 157: 144–153.
  • 28
    McWilliams RR, Gidey E, Fouassier L, Weed SA, Doctor RB. Characterization of an ankyrin repeat-containing Shank2 isoform (Shank2E) in liver epithelial cells. Biochem J 2004; 380: 181–191.
  • 29
    Sutherland E, Dixon BS, Leffert HL, Skally H, Zaccaro L, Simon FR. Biochemical localization of hepatic surface-membrane Na+,K+-ATPase activity depends on membrane lipid fluidity. Proc Natl Acad Sci U S A 1988; 85: 8673–8677.
  • 30
    Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ. Improved patch clamp techniques for high resolution recording from cells and cell-free membrane patches. Pfluegers Arch 1981; 391: 85–100.
  • 31
    Chang D, Hsieh PS, Dawson DC. Calcium: a program in basic for calculating the composition of solutions with specified free concentrations of calcium, magnesium and other divalent cations. Comput Biol Med 1988; 18: 351–366.
  • 32
    Fitz JG, Sostman A. Nucleotide receptors activate cation, potassium, and chloride currents in a liver cell line. Am J Physiol 1994; 266: G544–G553.
  • 33
    Lidofsky SD, Xie MH, Sostman A, Scharschmidt BF, Fitz JG. Vasopressin increases cytosolic sodium concentration in hepatocytes and activates calcium influx through cation-selective channels. J Biol Chem 1993; 268: 14632–14636.
  • 34
    Diaz ME, Trafford AW, Eisner DA. The effects of exogenous calcium buffers on the systolic calcium transient in rat ventricular myocytes. Biophys J 2001; 80: 1915–1925.
  • 35
    Auld A, Chen JL, Brereton HM, Wang YJ, Gregory RB, Barritt GJ. Store-operated Ca2+ inflow in Reuber hepatoma cells is inhibited by voltage-operated Ca2+ channel antagonists and, in contrast to freshly isolated hepatocytes, does not require a pertussis toxin-sensitive trimeric GTP-binding protein. Biochim Biophys Acta 2000; 1497: 11–26.
  • 36
    Fleig WE, Noetherfleig G, Fussgaenger R, Ditschuneit H. Modulation by a sulfonylurea of insulin-dependent glycogenesis, but not of insulin binding, in cultured rat hepatocytes—evidence for a postreceptor mechanism of action. Diabetes 1984; 33: 285–290.
  • 37
    Roman RM, Feranchak AP, Davison AK, Schwiebert EM, Fitz JG. Evidence for Gd(3+) inhibition of membrane ATP permeability and purinergic signaling. Am J Physiol 1999; 277: G1222–G1230.
  • 38
    Minguetti-Camara VC, Constantin J, Suzuki-Kemmelmeier F, Ishii-Iwamoto EL, Bracht A. Hepatic heterogeneity in the response to ATP studied in the bivascularly perfused rat liver. Mol Cell Biochem 1998; 179: 35–48.
  • 39
    Iizuka K, Miller B, Uyeda K. Deficiency of a carbohydrate-activated transcription factor, ChREBP, prevents obesity and improves plasma glucose control in leptin deficient (ob/ob) mice. Am J Physiol 2006; 291: E358–E364.
  • 40
    Liang L, Zsembery A, Schweibert EM. RNA interference targeted to multiple P2X receptor subtypes attenuates zinc-induced calcium entry. Am J Physiol 2005; 289: C388–C396.
  • 41
    Che M, Gatmaitan Z, Arias IM. Ectonucleotidases, purine nucleoside transporter, and function of the bile canalicular plasma membrane of the hepatocyte. FASEB J 1997; 11: 101–108.
  • 42
    Roman RM, Feranchak AP, Salter KD, Wang Y, Fitz JG. Endogenous ATP release regulates Cl- secretion in cultured human and rat biliary epithelial cells. Am J Physiol 1999; 276: G1391–G1400.
  • 43
    Torres GE, Egan TM, Voigt MM. Hetero-oligomeric assembly of P2X receptor subunits—specificities exist with regard to possible partners. J Biol Chem 1999; 274: 6653–6659.
View Full Article (HTML) Get PDF (317K)