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Supplementary material for this article can be found on the H EPATOLOGY Web site ( http://interscience.wiley.com/jpages/0270-9139/suppmat/index.html ).

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hep22163-FigS1.pdf24K Fig. S1. CNTFRα mRNA was ablated by CNTFRα-specific siRNA duplexes. 60 hours after transfection with three different CNTFRα-specific siRNA duplexes, the CNTFRα mRNA in SMMC-7721 cells was detected by quantitative PCR analysis. *P<0.01 versus control.
hep22163-FigS2.pdf29K Fig. S2. Kinase inhibitors were used to block CNTF-induced activation of multiple pathways. (A) SMMC-7721 cells were pretreated with MEK1/2 inhibitor U0126 (10 μM) for 2 hours. After treatment with CNTF (100 ng/mL) for 15 minutes, the cell extracts were analyzed by western blot using phospho-ERK1/2, ERK1/2 and β-actin antibodies. (B) SMMC-7721 cells were pretreated with PI3K inhibitor LY294002 (10 μM) for 2 hours. After treatment with CNTF (100 ng/mL) for 15 minutes, the cell extracts were analyzed by western blot using phospho-Akt, Akt and β-actin antibodies. (C) The SMMC-7721 cells were pretreated with JAK2/3 inhibitor AG490 (50 μM) for 2 hours. After treatment with CNTF (100 ng/mL) for 15 minutes, the cell extracts were analyzed by western blot using phospho-STAT3, STAT3 and β-actin antibodies.
hep22163-FigS3.pdf36K Fig. S3. siRNA-mediated knockdown of CNTFRα suppressed cell proliferation.SMMC-7721 cells were transfected with CNTFRα-siRNA duplixes. Cell proliferation assay (MTT) was performed for 6 days. *P<0.05, **P<0.01 versus control.

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