Potential conflict of interest: Nothing to report.
Article first published online: 18 APR 2008
Copyright © 2008 American Association for the Study of Liver Diseases
Volume 48, Issue 2, pages 607–616, August 2008
How to Cite
Geisler, F., Nagl, F., Mazur, P. K., Lee, M., Zimber-Strobl, U., Strobl, L. J., Radtke, F., Schmid, R. M. and Siveke, J. T. (2008), Liver-specific inactivation of Notch2, but not Notch1, compromises intrahepatic bile duct development in mice. Hepatology, 48: 607–616. doi: 10.1002/hep.22381
See Editorial on Page 358
- Issue published online: 29 JUL 2008
- Article first published online: 18 APR 2008
- Accepted manuscript online: 18 APR 2008 12:00AM EST
- Manuscript Accepted: 13 APR 2008
- Manuscript Received: 4 JAN 2008
- Kommission für Klinische Forschung of the Technical University of Munich. Grant Number: KKF 33-04
- Deutsche Forschungsgemeinschaft. Grant Number: STR-461/3-2
Additional Supporting Information may be found in the online version of this article.
|hep22381-SupplementaryFigure1Notch-deletion.tif||8507K||Supplementary Figure 1: AlbCre driven recombination of floxed alleles in embryonicN1N2 F/F AlbCreanimals. (A, B) X-Gal staining of liver sections obtained from E17.5 N1N2F/FRosa26AlbCre embryos. The outlined area in (A) is magnified in (B). (C) PCR with liver DNA isolated from E17.5 and P30 control andN1N2 F/F AlbCreanimals as indicated using recombination-specific primers for deleted Notch1 and Notch2 alleles. Upper panel: PCR detecting Cre recombinase including internal control.|
|hep22381-SupplementaryFigure2Notch-expression.tif||11714K||Supplementary Figure 2: Expression profile of Notch1 and Notch2 in livers of mice at P0 and P50. (A-D) Notch1 expression was analyzed by immunofluorescence using liver cryosections from transgenicNotch1-GFPanimals of indicated age using an anti-GFP (green) antibody. At P0 (A, B), Notch1 expression was most prominent in a number of cells with high nuclear-cytoplasmic ratio, most likely cells of the hematopoetic system (arrowheads in B). Notch1-expression could be detected in hepatocytes at P0 (A, B) and P50 (B,C) but was notably absent in biliary epithelial cells. Asterisks in (A), (B), and (D) mark lumina of bile ducts. (E,F) In contrast, X-Gal staining of livers fromNotch2 +/lacZ animals revealed strongest staining of developing bile ducts and ductal plate structures at P0 (E) and of bile ducts at P50 (F). Arrowheads in enlarged insets of (E) and (F) mark X-Gal positive bile ducts and ductal plate structures (E, right lower panel). Scale bar in (A): 25 μm for (A), 50 μm for (C). Scale bar in (E): 50 μm for (E), 200 μm for (F).|
|hep22381-SupplementaryTables.doc||48K||Supporting Information file hep22381-SupplementaryTables.doc|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.