Insulin induces calcium signals in the nucleus of rat hepatocytes†
Article first published online: 27 MAY 2008
Copyright © 2008 American Association for the Study of Liver Diseases
Volume 48, Issue 5, pages 1621–1631, November 2008
How to Cite
Rodrigues, M. A., Gomes, D. A., Andrade, V. A., Leite, M. F. and Nathanson, M. H. (2008), Insulin induces calcium signals in the nucleus of rat hepatocytes. Hepatology, 48: 1621–1631. doi: 10.1002/hep.22424
Potential conflict of interest: Nothing to report.
- Issue published online: 28 OCT 2008
- Article first published online: 27 MAY 2008
- Accepted manuscript online: 27 MAY 2008 12:00AM EST
- Manuscript Accepted: 11 MAY 2008
- Manuscript Received: 4 DEC 2007
- National Institutes of Health (NIH). Grant Numbers: DK57751, DK34989, DK45710
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação de Amparo à Pesquisa do Estado de Minas Gerais
- Howard Hughes Medical Institute
Insulin is an hepatic mitogen that promotes liver regeneration. Actions of insulin are mediated by the insulin receptor, which is a receptor tyrosine kinase. It is currently thought that signaling via the insulin receptor occurs at the plasma membrane, where it binds to insulin. Here we report that insulin induces calcium oscillations in isolated rat hepatocytes, and that these calcium signals depend upon activation of phospholipase C and the inositol 1,4,5-trisphosphate receptor, but not upon extracellular calcium. Furthermore, insulin-induced calcium signals occur in the nucleus, and are temporally associated with selective depletion of nuclear phosphatidylinositol bisphosphate and translocation of the insulin receptor to the nucleus. These findings suggest that the insulin receptor translocates to the nucleus to initiate nuclear, inositol 1,4,5-trisphosphate-mediated calcium signals in rat hepatocytes. This novel signaling mechanism may be responsible for insulin's effects on liver growth and regeneration. (HEPATOLOGY 2008.)
Insulin regulates a wide variety of biological functions in the liver, including glucose uptake,1 regulation of gene expression,2 and promotion of cell growth.3–5 The biological actions of insulin are initiated by binding to the insulin receptor, a heterotetrameric receptor tyrosine kinase (RTK) composed of two extracellular α-subunits and two transmembrane β-subunits.6 The α-subunit possesses insulin-binding activity whereas the β-subunit has intrinsic protein tyrosine kinase activity. Binding of insulin to the α-subunit of its receptor activates the protein tyrosine kinase and results in phosphorylation of tyrosine residues of the β-subunit and of several endogenous substrates. These substrates include proteins containing a src-homology 2 domain such as phosphatidylinositol 3-kinase and phospholipase C (PLC).7 PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2), generating two intracellular products: inositol 1,4,5-trisphosphate (InsP3), a universal calcium-mobilizing second messenger, and diacylglycerol, an activator of protein kinase C. Like insulin, Ca2+ also regulates glucose metabolism,8 gene expression,9, 10 and cell growth.11, 12 Although it has not been established how a single second messenger coordinates such diverse effects within a cell, there is increasing evidence that the spatial and temporal patterns of Ca2+ signals may determine their specificity. Ca2+ signaling patterns can vary in different regions of the cell, and increases in Ca2+ in the nucleus have specific biological effects that differ from the effects of increases in cytosolic Ca2+.9, 10, 13–15 The mechanisms and pathways that promote localized increases in free Ca2+ levels in the nucleus have not been entirely defined. It is currently thought that signaling via the insulin receptor occurs only at the plasma membrane, where it binds to insulin.16 Here we investigate whether and how insulin signaling occurs in the nucleus of hepatocytes, where its downstream messenger Ca2+ may act.
Materials and Methods
Cells and Cell Culture.
Hepatocytes were isolated from the livers of male Sprague-Dawley rats (190–200 g; Charles River Laboratories, Wilmington, MA) by collagenase perfusion as described.17 Primary hepatocytes were cultured at 37°C in 5% CO2/95% O2 in Williams' medium E containing 10% fetal bovine serum, 50 units/mL penicillin, and 50 g/mL streptomycin (Invitrogen, Carlsbad, CA) and plated on collagen-coated coverslips (50 μg/mL) (BD Biosciences, San Jose, CA). Hepatocytes were used 4–6 hours after isolation. Viability of the hepatocytes was greater than 85% and was measured by trypan blue exclusion.18, 19 SkHep1 cells, a human liver cancer cell line, were cultured at 37°C in 5% CO2 in Dulbecco's modified Eagle's medium (Invitrogen) containing 10% fetal bovine serum, 1 mM sodium pyruvate, 50 units/mL penicillin, and 50 g/mL streptomycin (Invitrogen).
Detection of Ca2+ Signals.
Nuclear and cytosolic Ca2+ were monitored in individual cells by time-lapse confocal microscopy, as described.14, 20 For Ca2+ imaging, cells were incubated with fluo-4/AM (6 μM) (Invitrogen) for 30 minutes at 37°C, then coverslips containing the cells were transferred to a custom-built perfusion chamber on the stage of a Zeiss LSM 510 confocal microscope (Thornwood, NY) and the perfusion chamber was maintained at 37°C. The cells were stimulated with insulin (1–500 nM) or vasopressin (10 nM) (Sigma, Saint Louis, MO). In selected experiments cells were perfused for 10 minutes with the PLC inhibitor U-73122 (1 μM) or pretreated for 30 minutes with the InsP3 receptor inhibitor xestospongin C (2.5 μM) (Sigma). Fluo-4 fluorescence was monitored using a 40×, 1.2 NA objective lens, and images were collected at a rate of 1–5 frames/second. Changes in fluorescence F were normalized by the initial fluorescence (F0) and were expressed as (F/F0) × 100%.11
InsP3 Buffer Constructs.
The InsP3 binding domain (residues 224–605) of the human type I InsP3 receptor was tagged with monomeric red fluorescent protein (mRFP) and then the nuclear localization signal was subcloned to generate the nuclear InsP3 buffer expression vector. The nuclear exclusion signal sequence derived from mitogen-activated protein kinase kinase 1 was subcloned in the InsP3 binding domain tagged with the mRFP construct to generate the cytoplasmic InsP3 buffer expression vector, as described.21
Primary hepatocyte immunoblots were performed as described.22 Briefly, cells were washed twice with ice-cold phosphate-buffered saline, harvested by scraping, and lysed in a lysis buffer (20 mM [4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid], pH 7.0, 10 mM KCl, 2 mM MgCl2, 0.5% Nonidet P-40). After incubation on ice for 10 minutes, the cells were homogenized by vortex. The homogenate was centrifuged at 1,500g for 5 minutes to sediment the nuclei. The supernatant was then centrifuged at a maximum speed of 16,100g for 20 minutes, and the resulting supernatant formed the non-nuclear fraction. The nuclear pellet was washed three times with lysis buffer to remove any contamination from cytoplasmic membranes, and the purity of the nuclei was confirmed by light microscopy. To extract nuclear proteins, the isolated nuclei were resuspended in NETN buffer (150 mM NaCl, 1 mM EDTA, 20 mM Tris-HCL, pH 8.0, 0.5% Nonidet P-40), and the mixture was sonicated briefly to aid nuclear lysis. Nuclear lysates were collected after centrifugation at 16,100g for 20 minutes at 4°C. Protease and phosphatase inhibitors (Sigma) were added to all buffers. Blots were visualized by enhanced chemiluminescence, and quantitatively analyzed using a GS-700 imaging densitometer. The purity of nuclear and non-nuclear fractions was confirmed using Lamin B1 (Abcam, Cambridge, MA) as a nuclear marker and α-Tubulin (Sigma) as a non-nuclear (cytosolic) marker.23 The phosphorylated form of the insulin receptor was detected by immunoprecipitation of the receptor, followed by blotting with a monoclonal antibody directed against phosphotyrosine residues (Millipore, Billerica, MA).
Detection of PIP2.
A PI(4,5)P2 Mass Strip Kit (Echelon, Salt Lake City, UT) was used for isolation and PIP2 detection. Isolated hepatocytes were starved in serum-free William's E medium for 3 hours. Cells were incubated without or with insulin (10 nM) for 10 minutes or vasopressin (10 nM) for 1 minute, and then the medium was aspirated and cellular material precipitated by the immediate addition of 3 mL ice-cold 0.5 M trichloroacetic acid. The lysis buffer was used to prepare the nuclear and non-nuclear cell fractions, as described above.22 Briefly, cell membranes were disrupted to release cytoplasmic contents. Intact nuclei were recovered from the cytoplasmic extract by centrifugation, and then the nuclei were washed with phosphate-buffered saline and precipitated with 3 mL ice-cold 0.5 M trichloroacetic acid. Isolation of lipids was performed according to manufacturer instructions and as described.24 The organic phase was collected into a clean tube and dried in a Speed Vac centrifuge. The pellet at this stage was faintly visible. The lipids were then resuspended by sonication in a cold water bath in 10 μL of CHCl3:methanol:H2O (1:2:0.8), and spotted onto nitrocellulose membrane strips prespotted with PI(4,5)P2 standards, PIP controls, and space for spotting unknown samples for probing with anti-PIP2 monoclonal antibody (Echelon) to specifically detect PIP2. Blots were visualized by enhanced chemiluminescence, and quantitatively analyzed using a GS-700 imaging densitometer (Bio-Rad, Hercules, CA).
Confocal immunofluorescence was performed as described.14, 20 Cells were double-labeled with a polyclonal antibody against insulin receptor B (BD Biosciences, CA), which is the predominant form of the receptor in hepatocytes,25 and a monoclonal antibody against the nuclear membrane marker Lamin B1, and then incubated with secondary antibodies conjugated to Alexa 488 and 555 (Invitrogen), respectively. Images were collected with a Zeiss LSM 510 confocal microscope using a 63×, 1.4 NA objective lens with excitation at 488 nm and observation at 505–550 nm to detect Alexa 488, and excitation at 543 nm and observation at 560–610 nm to detect Alexa 555.
Significance of changes in treatment groups relative to controls was determined by Student t test. Data are represented as mean ± standard error.
Insulin Induces Ca2+ Oscillations in Rat Hepatocytes.
To examine Ca2+ signaling induced by insulin, freshly isolated rat hepatocytes were stimulated with a range of insulin concentrations (0.1–100 nM) and observed by time-lapse confocal microscopy. Hepatocytes did not respond to 0.1 nM insulin (n = 30), but responded to all higher concentrations tested. The fraction of cells responding to insulin did not vary appreciably with increasing insulin concentrations; 41% of cells responded to stimulation with 1 nM insulin (Fig. 1A), 42% of cells responded to 10 nM insulin (Fig. 1B), and 56% of cells responded to maximal (100 nM) stimulation (Fig. 1C). Ca2+ oscillations were elicited in all responding cells stimulated with lower (1–10 nM) insulin concentrations, although higher insulin concentrations elicited Ca2+ oscillations in only 10% of responding cells, and instead elicited a sustained increase in Ca2+ in the remaining 46% of responding cells (data not shown). Moreover, the response to 100 nM and 500 nM insulin was similar, suggesting that these findings represent the full range of insulin's effect on Ca2+ signals in hepatocytes. The frequency of Ca2+ oscillations (∼5 mHz) was similar regardless of the insulin concentration. These findings show that insulin, like other Ca2+ agonists such as vasopressin, phenylephrine, angiotensin, and adenosine triphosphate,26–28 induces Ca2+ signals in hepatocytes that tend to be oscillatory at lower concentrations but can instead be sustained at higher concentrations. However, the frequency of insulin-induced Ca2+ oscillations was lower than has typically been reported for other agonists such as phenylephrine (10–50 mHz)26, 27 and vasopressin (10–35 mHz).26, 28 In addition, maximal concentrations of these other agonists generally elicit Ca2+ signals in >90% of hepatocytes,26–28 whereas insulin elicited Ca2+ signals in a much lower fraction of cells. Moreover, we stimulated cells with vasopressin (10 nM) and those results confirmed that ∼98% of cells responded to that agonist, even though only half of the cells responded to insulin under the same experimental conditions. Vasopressin also induced a greater peak in fluorescence than what was observed in response to insulin stimulation (Fig. 1D). These findings demonstrate that insulin induces Ca2+ signals in hepatocytes, including Ca2+ oscillations, but that certain characteristics of these signals differ from what is elicited by stimulation of G protein–coupled receptors.
Insulin-Induced Ca2+ Signals Are Mediated by InsP3.
Several maneuvers were performed to determine the mechanism by which insulin increases Ca2+ in hepatocytes. To determine the source of the Ca2+, cells were stimulated in Ca2+-free medium. Insulin induced Ca2+ oscillations even in Ca2+-free medium (Fig. 2A), and Ca2+ signals were elicited in a similar fraction of cells regardless of the presence of extracellular Ca2+ (Fig. 2B). These findings demonstrate that insulin increases cytoplasmic Ca2+ by mobilizing intracellular Ca2+ stores. Most RTKs increase Ca2+ by activation of PLCγ, which forms InsP3 to bind to and release Ca2+ from InsP3 receptors in the endoplasmic reticulum.29 Therefore, we stimulated hepatocytes with insulin in the presence of either the PLC inhibitor U-7312230 or the InsP3 receptor inhibitor xestospongin C.31 Both U-73122 (Fig. 3A,B) and xestospongin C (Fig. 3C,D) eliminated insulin-induced Ca2+ signals in hepatocytes. Together, these findings suggest that insulin increases Ca2+ in hepatocytes through PLC- and InsP3-mediated release of intracellular Ca2+ stores.
Insulin-Induced Ca2+ Signals Begin in the Nucleus.
Ca2+ signals in the nucleus and cytoplasm were monitored simultaneously in hepatocytes (n > 30). The signals often had a similar temporal profile in both compartments (Fig. 4A), but the Ca2+ increase in the nucleus preceded the cytoplasmic increase in some cells, while in other cells an isolated increase in Ca2+ in the nucleus was observed (Fig. 4B). The kinetics of vasopressin-induced Ca2+ signals differed from this in two ways. First, insulin-induced signals often took up to 50 seconds from the time of onset to reach their peak amplitude (Figs. 1A-C and 4A), whereas the rise time of vasopressin-induced signals always was much shorter (∼1 second; Fig. 1D), similar to what has been reported.18 Second, vasopressin-induced Ca2+ signals always began in the cytoplasm rather than the nucleus (Fig. 4C). These findings indicate that the subcellular kinetics of insulin-induced Ca2+ signals differ fundamentally from the kinetics of Ca2+ signals induced by vasopressin, which in turn suggests that insulin may increase Ca2+ through a mechanism based in the nucleus rather than in the cytoplasm.
The Insulin Receptor Translocates to the Nucleus.
Ca2+ signals are initiated in hepatocytes when PIP2 is hydrolyzed to form InsP3.18 Both the nucleus and the cytoplasm contain the machinery needed to form InsP3-mediated Ca2+ signals, including PLC, PIP2, and the InsP3 receptor,32 so we examined the effects of insulin on total cellular and nuclear pools of PIP2. Insulin reduced the nuclear pool by 38.9 ± 7.1% (P < 0.05) without significantly reducing total cellular PIP2 (Fig. 5). For comparison, vasopressin reduced total cellular PIP2 by 35.2 ± 7.8% (P < 0.05) without significantly reducing nuclear PIP2. To demonstrate more directly that the insulin receptor forms InsP3 in the nucleus, we targeted the ligand binding domain (residues 224–605) of the type 1 InsP3 receptor33 to the cytoplasm or nucleus using a nuclear exclusion signal or nuclear localization signal sequence, respectively, plus mRFP to verify localization.21 These targeted InsP3 buffer constructs were expressed in the SkHep1 liver cell line, to circumvent technical difficulties associated with transient transfection of primary hepatocytes. It has previously been shown that the cytoplasmic but not the nuclear InsP3 buffer blocks vasopressin-induced Ca2+ signals in SkHep1 cells, reflecting the fact that G protein-coupled receptors such as the vasopressin V1a receptor activate PLC and form InsP3 at the plasma membrane.21 In contrast, Ca2+ signals induced by insulin (100 nM) were nearly abolished in cells expressing the nuclear InsP3 buffer (P < 0.005), but were not affected by expression of the cytoplasmic buffer (Fig. 6). Together, these results show that insulin hydrolyzes PIP2 and increases InsP3 only in the nucleus, and that Ca2+ signals throughout the cell result from this. To investigate why insulin preferentially forms InsP3 and increases Ca2+ in the nucleus, we examined the location of the insulin receptor during cell stimulation. Immunoblots of non-nuclear and nuclear fractions showed that the insulin receptor was in the non-nuclear fraction of hepatocytes prior to stimulation with insulin. However, the receptor appeared in the nuclear fraction within 2.5 minutes of stimulation, and was detectable within the nucleus until 20 minutes after stimulation (Fig. 7A,B). Similarly, the phosphorylated (active) form of the insulin receptor was absent from the nucleus of hepatocytes prior to stimulation with insulin, but was detected there afterwards (Fig. 7C). To confirm the immunoblot findings, confocal immunofluorescence microscopy was used to monitor the subcellular distribution of the insulin receptor. Confocal imaging demonstrated that the insulin receptor was at the plasma membrane or within the cytoplasm but absent from the nucleus prior to stimulation (Fig. 8A, top panels). Within 5 minutes of exposure to 10 nM insulin, the insulin receptor could also be detected at the nuclear envelope and within the nuclear interior (Fig. 8A, bottom panels). Three-dimensional (3D) reconstruction of serial confocal immunofluorescence images confirmed that the receptor could be identified within the nuclear interior of cells stimulated with insulin (Fig. 8B). Together, these findings demonstrate that stimulation of hepatocytes with insulin induces the insulin receptor to translocate to the nucleus, and this is associated with selective hydrolysis of nuclear PIP2 and formation of InsP3-dependent Ca2+ signals within the nucleus.
Insulin is a potent mitogen for hepatocytes in vitro4 and also plays a role in liver regeneration in vivo.34 Insulin also plays an essential role in the growth and proliferation of hepatocytes in certain cell culture systems.35 Insulin acts through the insulin receptor, which is a RTK, and evidence from other RTKs suggests that translocation to the nucleus may be a common feature for this class of receptors. A number of RTKs have been found in the nucleus, including receptors for growth hormone, several cytokines, epidermal growth factor (EGF), hepatocyte growth factor, and fibroblast growth factor (FGF).21, 36, 37 Phosphorylated EGF receptor can be found in the nucleus within 1–2 minutes of stimulation with EGF, and reaches peak levels within 15 minutes.36 Phosphorylated hepatocyte growth factor receptor (c-met) appears in the nucleus within a similar time frame after stimulation with hepatocyte growth factor, and its appearance there has been linked to intranuclear formation of InsP3 and initiation of Ca2+ signals within the nucleus.21 Translocation of the FGF receptor to the nucleus occurs over a longer time scale, reaching peak amounts after 3–4 hours.37 Although these previous studies have demonstrated that RTKs can translocate to the nucleus in cell lines, the current work provides evidence that this also occurs in primary hepatocytes. Intranuclear RTKs can serve functional effects as well. For example, EGF receptors in the nucleus act as a transcription factor that promotes expression of cyclin D136 and COX-2,38 each of which may contribute to the mitogenic effects of EGF. The mechanism by which RTKs reach the nucleus is not known, although transport of the FGF receptor to the nucleus depends on importin β, rather than the presence of a nuclear localization sequence on either the receptor or its ligand,37 and transport of c-met to the nucleus depends upon both importin β and the adaptor protein GRB2-associated binding protein 1 (Gab1).21 Early studies based on binding of radiolabeled insulin to nuclear membranes,39 plus autoradiographic studies of hepatocytes and hepatocyte lysates using photolabeled insulin receptors,40 suggested that the insulin receptor can be intranuclear. This conclusion was questioned in later work using immunoblot and immunoelectron microscopic techniques,41 which had led many to conclude instead that the insulin receptor does not translocate to the nucleus.42 Similarly, previous evidence had suggested that the insulin receptor does not activate PLC, leading to the widely held conclusion that insulin does not stimulate the PLC/InsP3/Ca2+ signaling pathway.43, 44 However, recent studies have shown an increase in InsP3 in rat epididymal cells stimulated with insulin,45 as well as an increase in PLC activity in insulin-stimulated adipocytes.46 Moreover, PLCγ coprecipitates with the insulin receptor, providing additional evidence that this receptor induces phospholipid hydrolysis.7 Finally, insulin has been reported to increase cytosolic Ca2+ in primary hepatocytes by triggering Ca2+ influx,47 but the current work provides evidence that insulin instead mobilizes intracellular Ca2+ stores in hepatocytes, through a PLC-dependent and InsP3-dependent mechanism. The current findings provide both structural and functional evidence that the insulin receptor moves to and acts within the nucleus in hepatocytes. Structural evidence includes immunoblots showing that total as well as phosphorylated insulin receptor accumulates in the nucleus, plus confocal immunofluorescence localization of the receptor within the nucleus. Functional evidence includes studies showing that insulin selectively hydrolyzes the nuclear pool of PIP2, plus Ca2+ imaging studies showing that insulin-induced Ca2+ signals can begin in the nucleus, and that these signals depend on intranuclear rather than cytoplasmic InsP3. Thus, previous studies plus the current work together suggest that insulin induces its receptor to move to the nucleus in hepatocytes, and this translocation is associated with PLC-mediated hydrolysis of nuclear PIP2, leading to formation of InsP3-mediated Ca2+ signals. Because Ca2+ signals within the nucleus are particularly important for cell growth,11 the effect of insulin on nuclear Ca2+ signaling may explain insulin's action as a mitogen. The metabolic effects of insulin in the liver are mediated by Akt/protein kinase B, and these effects are enhanced in the liver-specific Gab1 knockout mouse.48 Since Gab1 may mediate nuclear translocation of RTKs,21 this suggests that the metabolic effects of insulin may be mediated by the non-nuclear insulin receptor, while the effects of insulin on growth and regeneration may be mediated by the insulin receptor that reaches the nucleus.
There is increasing evidence that the subcellular pattern of Ca2+ signals dictates the cellular effects of this second messenger. The InsP3 receptor is the only intracellular Ca2+ release channel in hepatocytes,18 so the subcellular distribution of this receptor determines the form of Ca2+ signals in these cells. For example, the type II InsP3 receptor, which is the principle isoform in hepatocytes, is most concentrated in the region of the endoplasmic reticulum beneath the canalicular membrane.18, 19 Agonists such as vasopressin, angiotensin, or adenosine triphosphate increase InsP3 in the cytosol, and so the resulting Ca2+ signal takes the form of a Ca2+ wave that begins in the canalicular region, where the InsP3 receptor is most concentrated.18 This Ca2+ wave directs exocytosis49 and fluid and electrolyte secretion.50 Reduced expression of InsP3 receptors in hepatocytes impairs the formation of Ca2+ waves,51 but simple redistribution of the receptors away from the canalicular region impairs Ca2+ wave formation as well.19 This subcellular organization of the Ca2+ signaling machinery is relevant for the regulation of secretion, because treatment of cholangiocytes with small interfering RNA to decrease expression of apical InsP3 receptors in these cells results in impaired bicarbonate secretion.52 Expression of apical InsP3 receptors is also decreased or absent in bile ducts of patients with cholestatic disorders such as primary biliary cirrhosis, sclerosing cholangitis, and biliary atresia,53 although it has not yet been established that this loss of InsP3 receptors is responsible for the development of cholestasis in these disorders. Localization of InsP3 receptors to other microdomains can affect cell function as well. For example, the type III InsP3 receptor colocalizes more effectively than either the type I or II isoform of the receptor with mitochondria.54 This is associated with more efficient transmission of Ca2+ signals into the mitochondria, which in turn is more effective at inducing apoptosis.54 A number of Ca2+-mediated events occur in the nucleus; including: activation of cyclic adenosine monophosphate response element-binding transcription factor10 and the Elk-1 transcription factor9; translocation of nuclear protein kinase C to the region of the nuclear envelope14; and regulation of progression of the cell cycle through prophase.11 Although Ca2+ can spread passively from the cytosol into the nucleus under certain circumstances,55, 56 intranuclear InsP3 can increase Ca2+ directly within the nucleus as well, in both isolated nuclei and in nuclei within intact cells.14, 57 This is because the nuclear envelope57 and the nucleoplasmic reticulum14 both express InsP3 receptors, and these receptors can release Ca2+ into the nucleoplasm. How much InsP3 receptor is expressed in the nucleus? Although immunofluorescence studies suggest that the InsP3 receptor in hepatocytes is most concentrated in the pericanalicular region,18 quantitative immunoblots show that the ratio of nuclear:cytosolic InsP3 receptors is nearly 20:1.14 This ratio reflects InsP3 receptor concentration relative to other proteins in each compartment, but since there is presumably much less total protein per unit volume in the nucleus than in the cytoplasm, this may explain why immunofluorescence studies instead suggest that there is more InsP3 receptor in the cytoplasm. In any case, several mechanisms have been identified to control Ca2+ release from nuclear InsP3 receptors. The three InsP3 receptor isoforms have distinct sensitivities to InsP3, so targeting a more sensitive isoform to the nucleus will enable InsP3-mediated Ca2+ signals to occur preferentially in the nucleus, relative to the cytosol.20 Alternatively, selective hydrolysis of the nuclear pool of PIP2 will lead to local intranuclear formation of InsP3, so that Ca2+ will be released preferentially from nuclear InsP3 receptors. In particular, RTKs may selectively activate nuclear isoforms of PLC, particularly PLCβ1, and may also induce PLCγ1 to translocate to the nucleus.58, 59 The current work suggests that the insulin receptor may also act in this fashion. Although insulin-induced Ca2+ signals begin before peak accumulation of the insulin receptor occurs within the nucleus, there is likely a threshold relationship rather than a linear relationship between accumulation of insulin receptor within the nucleus and triggering of Ca2+ signals, just as there is a threshold, “all-or-none” relationship between accumulation of InsP3 and initiation of Ca2+ signals in the cytoplasm.60 Therefore, although the increase in intranuclear insulin receptor does not become measurable for several minutes, smaller amounts, especially of the phosphorylated receptor, may be sufficient to generate enough InsP3 to initiate Ca2+ signals. Further work is needed to determine the mechanism by which the insulin receptor moves to the nucleus in hepatocytes and to demonstrate that this is responsible for insulin's mitogenic effects.
We thank Kathy Harry for hepatocyte isolations.
- 29The polyphosphoinositide cycle exists in the nuclei of Swiss 3T3 cells under the control of a receptor (for IGF-I) in the plasma membrane, and stimulation of the cycle increases nuclear diacylglycerol and apparently induces translocation of protein kinase C to the nucleus. EMBO J 1991; 10: 3207–3214., , .