RNA-binding protein insulin-like growth factor II mRNA-binding protein 3 expression promotes tumor invasion and predicts early recurrence and poor prognosis in hepatocellular carcinoma†
Article first published online: 9 JUN 2008
Copyright © 2008 American Association for the Study of Liver Diseases
Volume 48, Issue 4, pages 1118–1127, October 2008
How to Cite
Jeng, Y.-M., Chang, C.-C., Hu, F.-C., Chou, H.-Y. E., Kao, H.-L., Wang, T.-H. and Hsu, H.-C. (2008), RNA-binding protein insulin-like growth factor II mRNA-binding protein 3 expression promotes tumor invasion and predicts early recurrence and poor prognosis in hepatocellular carcinoma. Hepatology, 48: 1118–1127. doi: 10.1002/hep.22459
Potential conflict of interest: Nothing to report.
- Issue published online: 26 SEP 2008
- Article first published online: 9 JUN 2008
- Accepted manuscript online: 9 JUN 2008 12:00AM EST
- Manuscript Accepted: 29 MAY 2008
- Manuscript Received: 19 FEB 2008
- Physician Scientist Award. Grant Number: PS9304
- National Health Research Institute, Taiwan
Additional Supporting Information may be found in the online version of this article.
|hep22459-SupplementaryFigure1.tif||881K||Supplementary Figure 1.Correlation of mRNA expression and immunohistochemical staining of IMP3 in 40 nontumorous liver tissues and 114 HCCs. Real-time RT-PCR was done to determine the amount of IMP3 in tissue samples. The RNA amount was highly correlated with the extent of immunohistochemical staining in a dose-dependent manner. All P value of the differences between different group were <0.05. L: nontumorous liver parenchyma, 0+: tumors without IMP3 expression, 1+, 2+, 3+: tumors with 1+, 2+, 3+ IMP3 expression by immunohistochemistry.|
|hep22459-SupplementaryFigure2.tif||1619K||Supplementary Figure 2.Expression of IMP3 in liver cancer cell lines.|
|hep22459-SupplementaryFigure3.tif||11343K||Supplementary Figure 3.Effect of IMP3 expression on breast cancer cell line MDA-MB-231. pQCXIP-IMP3 and control vector were transduced into MDA-MB-231 cells by retrovirus. Compared with those with empty vector (A), MDA-MB-231 cells with IMP3 overexpression invaded through Matrigel more efficiently (B). (C) Statistical analysis of three wells for each group in one experiment.|
|hep22459-SupplementaryFigure4.tif||9165K||Supplementary Figure 4.Invadopodia formation in IMP3 depleted HA22T cells. After treated with siRNA for 3 days, the cells were plated on slides coated with fibronectin. Invadopodia was shown by colocalization of phosphotyrosine (green) and actin (red). (A) Control cells. (B) IMP3 depleted cells. 200 cells in each slide were counted to determine the percentage of cells with invadopodia. As shown in (C), the percentage of cells with invadopodia was similar in IMP3-depleted cells and control cells.|
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