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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
HEP_22531_SupportingFigure1.tif312KSupp Fig.1 Osteopontin (OPN) expression levels in HCC cell lines with different metastatic potentials analyzed by Western blot. GAPDH was used as an internal control. Amount of OPN is indicated as relative fold. OPN expression level was closely related to the metastatic potentials.
HEP_22531_SupportingFigure2.tif10226KSuppFig.2 The effect of lentiviral-mediated miRNA against OPN on in vitro proliferation and invasion of MHCC97-H cells.
HEP_22531_SupportingFigure3.tif1518KSuppFig.3 The lentiviral-mediated miRNA against OPN did not have any significant effect on the HepG2 cells.
HEP_22531_SupportingFigure4.tif5161KSuppFig.4 Visual and fluorescent images of subcutaneous tumors in nude mice implanted with HCCLM3 cells.
HEP_22531_SupportingFigure5.tif8244KSuppFig. 5 The comparison of lung metastases between the mock controls mice with implantation of HCCLM3 cells in the thoracic area and those with HCCCLM3 cells implanted in the rear abdominal area.
HEP_22531_SupportingFigure6.tif1231KSuppFig.6 The protein levels of IFN-induced gene (P-eIF2α) in the HCCLM3 cells.
HEP_22531_SupportingFigure7.tif3938KSuppFig. 7 The expression levels of OPN, pMEK, pERK1/2, MMP-2, uPA and MMP7hyphen;9 detected in the tumors at the end of the in vivo experiments.

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