SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
HEP_22715_sm_SupFig1.tif8363KSupporting Fig. 1. Isolation of CD56+ T and CD56− T cells from PBMCs. The purity of isolated cell population was determined by flow cytometry.
HEP_22715_sm_SupFig2.tif4271KSupporting Fig. 2. CD56+ T cells or supernatant (SN) suppress HCV replication in Huh 7.5.1 cells. (A) Co-culture of HCV infected-Huh7.5.1 cells with CD56− T or CD56+ T cells via a trans-well system. HCV JFH-1-infected Huh7.5.1 cells (day 3 post infection) were plated in the low compartment of a 24-well plate, while the different numbers of activated CD56− T or CD56+ T cells were added in the top compartment as indicated. The cultures were supplemented with IL-12 (20ng/mL) to maintain the activated status of T cells. After 48h co-culture, total cellular RNA extracted from Huh7.5.1 cells was subjected to the real-time RT-PCR for HCV and GAPDH RNA quantification. (B) Effect of CD56− T and CD56+ T cells SN on HCV replication in Huh7.5.1 cells. HCV JFH-1-infected Huh7.5.1 cells (day 3 post infection) were cultured in the presence or absence of CD56− T or CD56+ T SN at indicated concentrations for 48h. Total cellular RNA extracted from Huh7.5.1 cells was subjected to the real-time RT-PCR for HCV and GAPDH RNA quantification. The data are expressed as HCV RNA levels relative (%) to control (without T cells or SN treatment, which is defined as 100). The results are mean SD of triplicate cultures, representative of three experiments (*P < 0.05, **P < 0.01).
HEP_22715_sm_SupFig3.tif4935KSupporting Fig. 3. IFN-γ production from activated CD56− T and CD56+ T cells. CD56− T and CD56+ T cells isolated from same donors (n=3) were stimulated with MACSiBead particles loaded with CD2, CD3 and CD28 antibodies for 24h and 48h. The supernatants were collected and analyzed for IFN-γ protein by ELISA assay.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.