CD56+ T cells inhibit hepatitis C virus replication in human hepatocytes†
Article first published online: 5 NOV 2008
Copyright © 2008 American Association for the Study of Liver Diseases
Volume 49, Issue 3, pages 753–762, March 2009
How to Cite
Ye, L., Wang, X., Wang, S., Wang, Y., Song, L., Hou, W., Zhou, L., Li, H. and Ho, W. (2009), CD56+ T cells inhibit hepatitis C virus replication in human hepatocytes. Hepatology, 49: 753–762. doi: 10.1002/hep.22715
Potential conflict of interest: Nothing to report.
- Issue published online: 24 FEB 2009
- Article first published online: 5 NOV 2008
- Accepted manuscript online: 5 NOV 2008 12:00AM EST
- Manuscript Accepted: 21 OCT 2008
- Manuscript Received: 18 MAY 2008
- National Institutes of Health. Grant Numbers: DA 12815, DA 22177
- Foerderer Murray Award
Additional Supporting Information may be found in the online version of this article.
|HEP_22715_sm_SupFig1.tif||8363K||Supporting Fig. 1. Isolation of CD56+ T and CD56− T cells from PBMCs. The purity of isolated cell population was determined by flow cytometry.|
|HEP_22715_sm_SupFig2.tif||4271K||Supporting Fig. 2. CD56+ T cells or supernatant (SN) suppress HCV replication in Huh 7.5.1 cells. (A) Co-culture of HCV infected-Huh7.5.1 cells with CD56− T or CD56+ T cells via a trans-well system. HCV JFH-1-infected Huh7.5.1 cells (day 3 post infection) were plated in the low compartment of a 24-well plate, while the different numbers of activated CD56− T or CD56+ T cells were added in the top compartment as indicated. The cultures were supplemented with IL-12 (20ng/mL) to maintain the activated status of T cells. After 48h co-culture, total cellular RNA extracted from Huh7.5.1 cells was subjected to the real-time RT-PCR for HCV and GAPDH RNA quantification. (B) Effect of CD56− T and CD56+ T cells SN on HCV replication in Huh7.5.1 cells. HCV JFH-1-infected Huh7.5.1 cells (day 3 post infection) were cultured in the presence or absence of CD56− T or CD56+ T SN at indicated concentrations for 48h. Total cellular RNA extracted from Huh7.5.1 cells was subjected to the real-time RT-PCR for HCV and GAPDH RNA quantification. The data are expressed as HCV RNA levels relative (%) to control (without T cells or SN treatment, which is defined as 100). The results are mean SD of triplicate cultures, representative of three experiments (*P < 0.05, **P < 0.01).|
|HEP_22715_sm_SupFig3.tif||4935K||Supporting Fig. 3. IFN-γ production from activated CD56− T and CD56+ T cells. CD56− T and CD56+ T cells isolated from same donors (n=3) were stimulated with MACSiBead particles loaded with CD2, CD3 and CD28 antibodies for 24h and 48h. The supernatants were collected and analyzed for IFN-γ protein by ELISA assay.|
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