NKG2D–retinoic acid early inducible-1 recognition between natural killer cells and kupffer cells in a novel murine natural killer cell–dependent fulminant hepatitis

Authors

  • Xin Hou,

    1. Institute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, China
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  • Rongbin Zhou,

    1. Institute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, China
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  • Haiming Wei,

    1. Institute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, China
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  • Rui Sun,

    1. Institute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, China
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  • Zhigang Tian

    Corresponding author
    1. Institute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, China
    • School of Life Sciences, University of Science and Technology of China, 443 Huangshan Road, Hefei, Anhui 230027, China
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    • fax: 86-551-360-6783


  • Potential conflict of interest: Nothing to report.

Abstract

Increasing evidence suggests the contribution of natural killer (NK) cells to pathogenesis of human hepatitis, but the detailed mechanisms have yet to be clearly elucidated. In this study, injection of polyinosinic:polycytidylic acid (poly I:C) and D-galactosamine (D-GalN) was used to establish a novel murine fulminant hepatitis model: results showed that predepletion of either NK cells or Kupffer cells could completely abolish the liver injury. Injection of poly I:C/D-GalN into mice could promote tumor necrosis factor-α production and surface retinoic acid early inducible-1 (Rae1) protein expression by Kupffer cells, which then activated NK cells to produce interferon-γ via NKG2D-Rae1 recognition. NK cell–derived interferon-γ and Kupffer cell–derived tumor necrosis factor-α synergistically mediated the severe liver injury. Moreover, Kupffer cell–derived interleukin-12 and interleukin-18 were also found to improve cross talk between NK cells and Kupffer cells. Conclusion: These results provide the first in vivo evidence that NKG2D/ligand interaction is involved in the synergic effects of NK cells and Kupffer cells on acute liver injury. (HEPATOLOGY 2009.)

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