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Additional Supporting Information may be found in the online version of this article.

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HEP_22849_sm_SupDoct.doc39KSuppporting Information Materials and Methods
HEP_22849_sm_SupFig1.tif69KSupporting Fig. 1. A) Northern blot analysis: detection of HCV RNA in cells- expressing the full length HCV cDNA. Approximately 20 μg of total cellular RNA extracted from HepG2-HCV, HepG2-vector, Huh7-HCV, and Huh7-vector were electrophoresed in agrose gels and transferred onto nylon membrane, blots were hybridized with cDNA probe spanning from nucleotides 350 to 3500 of the HCV genome genotype 4a. The equal loading of total RNA was judged by GAPDH. B) Western blot analysis: detection of structural core (i), E1(ii) and E2 (iii), and non-structural NS3 (iv), NS4B (v), NS5A (vi) and NS5B (vii) proteins in both HepG2-HCV and Huh7-HCV transfectants. Whereas, β-actin (viii) was used as internal control for loading and transfer. C) Western blots analysis of TGF-β2 and VEGF in cells-expressing the full-length HCV cDNA in hepatoma cells. β-actin was used as internal control for loading und transfer. D) ELISA of TGF-β2 and VEGF production in culture media of hepatoma cells-expressing the full-length HCV cDNA. Data are averages ±S.D.of three independent experiments performed in duplicate.
HEP_22849_sm_SupFig2.tif132KSupporting Fig. 2. Production of TGF-β2 and VEGF, during HCV-infection, is induced by HCV core protein. ELISA analysis demonstrating the production of both TGF-β2 and VEGF in hepatoma cell lines by the constitutively expression of the full-length HCV cDNA, or by the regulated expression of HCV core, but not by the regulated expression of HCV NS3 protein. Data are averages ±S.D. of three independent experiments performed in duplicate.
HEP_22849_sm_SupFig3A.tif94KSupporting Fig. 3A. EMSA analysis demonstrating induction of the DNA-binding activity of HIF-1α by either the expression of the full-length HCV cDNA or by the expression HCV core in hepatoma cell lines. The treatment of HepG2-core, Huh7-core, HepG2-HCV, or Huh7-HCV transfectants by PX-487 blocked the DNA-binding activity of HIF-1α induced by the expression of HCV core or by the expression of the full-length HCV cDNA. Data are representative of three independent experiments.
HEP_22849_sm_SupFig3B.tif76KSupporting Fig. 3B. EMSA analysis demonstrating induction of the DNA-binding activity of HIF-1α by either the expression of the full-length HCV cDNA or by the expression HCV core in hepatoma cell lines. The treatment of HepG2-core, Huh7-core, HepG2-HCV, or Huh7-HCV transfectants by PX-487 blocked the DNA-binding activity of HIF-1α induced by the expression of HCV core or by the expression of the full-length HCV cDNA. Data are representative of three independent experiments.
HEP_22849_sm_SupFig3C.tif113KSupporting Fig. 3C. EMSA analysis demonstrating induction of the DNA-binding activity of HIF-1α by either the expression of the full-length HCV cDNA or by the expression HCV core in hepatoma cell lines. The treatment of HepG2-core, Huh7-core, HepG2-HCV, or Huh7-HCV transfectants by PX-487 blocked the DNA-binding activity of HIF-1α induced by the expression of HCV core or by the expression of the full-length HCV cDNA. Data are representative of three independent experiments.
HEP_22849_sm_SupFig3D.tif89KSupporting Fig. 3D. EMSA analysis demonstrating induction of the DNA-binding activity of HIF-1α by either the expression of the full-length HCV cDNA or by the expression HCV core in hepatoma cell lines. The treatment of HepG2-core, Huh7-core, HepG2-HCV, or Huh7-HCV transfectants by PX-487 blocked the DNA-binding activity of HIF-1α induced by the expression of HCV core or by the expression of the full-length HCV cDNA. Data are representative of three independent experiments.

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