Novel mechanism of C-reactive protein for enhancing mouse liver innate immunity†
Article first published online: 7 APR 2009
Copyright © 2009 American Association for the Study of Liver Diseases
Volume 49, Issue 6, pages 2044–2054, June 2009
How to Cite
Inatsu, A., Kinoshita, M., Nakashima, H., Shimizu, J., Saitoh, D., Tamai, S. and Seki, S. (2009), Novel mechanism of C-reactive protein for enhancing mouse liver innate immunity. Hepatology, 49: 2044–2054. doi: 10.1002/hep.22888
Potential conflict of interest: Nothing to report.
- Issue published online: 28 MAY 2009
- Article first published online: 7 APR 2009
- Accepted manuscript online: 7 APR 2009 12:00AM EST
- Manuscript Accepted: 26 JAN 2009
- Manuscript Received: 10 OCT 2008
- Special Research Program (host stress responses to internal and external factors) from the National Defense Medical College
Additional Supporting Information may be found in the online version of this article.
|HEP_22888_sm_SupplFig1.tif||91K||Supporting Fig. 1. The effect of synthetic CRP pretreatment on the phagocytotic activity of Kupffer cells after lethal E. coli infection in mice. CRP/PBS-pretreated mice (1 h before) were injected i.v. with FITC-microspheres 1 h after lethal E.coli injection. Liver MNCs were obtained from the mice 20 min after the microsphere injection to evaluate microsphere-phagocytosis by Kupffer cells. The data are representatives from three mice with similar results in each group.|
|HEP_22888_sm_SupplFig2.tif||67K||Supporting Fig. 2. The effect of synthetic CRP pretreatment on the generalized Shwartzman reaction. Mice were injected i.p. with IL-12 and, 16 h later, LPS was injected i.v.. They were pretreated with CRP (50, 250, or 500 μg) or PBS 1 h before LPS injection. The effect of CRP pretreatment on the survival rate (A) and the levels of serum TNF (B), and serum IFN-γ (C) after LPS challenge. Data are mean ± SE from ten mice in each group. * P<0.01, † P<0.05 vs. other groups.|
|HEP_22888_sm_SupplFig3.tif||49K||Supporting Fig. 3. The effect of synthetic CRP post-treatment on mouse survival after lethal E. coli challenge. Mice were challenged with the lethal dose of E. coli; after 1 h, they were injected i.v. with CRP/PBS. Survival rate are shown. N=10 in each group, *P<0.01.|
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