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HEP_22933_sm_SupFig1.tif124KFig. 1. After treatment with 5-FU (20 μg/ml) for 0, 24, 36 hrs, pro-caspase 8 (57 kDa) and the cleaved casp 8 (43/41 kDa) were detected in Vec-7703 and CHD1L-7703 cells. β-actin was used as a loading control.
HEP_22933_sm_SupFig2.tif101KFig. 2. (A) Northern blot analysis showed that siRNA against CHD1L effectively inhibited CHD1L expression in CHD1L-7703 cells, while no such silencing was observed with control siRNA against the GFP gene. 28S rRNA was used an internal control. (B) The apoptotic index after STS treatment, as detected by TUNEL assay, was compared between CHD1L-7703 cells treated with CHD1L-siRNA or GFP-siRNA. Bars indicate the averages of triplicate samples ± SD (**, P < 0.001, Student's t-test).
HEP_22933_sm_SupFig3.tif66KFig. 3. Western blot analysis showed that the cleavages of Casp 9, Casp 3 and PARP was substantially prohibited in CHD1L/300-897/7703 cells, but not in CHD1L/1-600/7703 cells. β-actin was used as a loading control.
HEP_22933_sm_Suptext.doc25KSupplementary Data

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