Steatohepatitis/Metabolic Liver Disease
Specific role for acyl CoA:Diacylglycerol acyltransferase 1 (Dgat1) in hepatic steatosis due to exogenous fatty acids†
Version of Record online: 19 MAR 2009
Copyright © 2009 American Association for the Study of Liver Diseases
Volume 50, Issue 2, pages 434–442, August 2009
How to Cite
Villanueva, C. J., Monetti, M., Shih, M., Zhou, P., Watkins, S. M., Bhanot, S. and Farese, R. V. (2009), Specific role for acyl CoA:Diacylglycerol acyltransferase 1 (Dgat1) in hepatic steatosis due to exogenous fatty acids. Hepatology, 50: 434–442. doi: 10.1002/hep.22980
Potential conflict of interest: Nothing to report.
- Issue online: 29 JUL 2009
- Version of Record online: 19 MAR 2009
- Accepted manuscript online: 19 MAR 2009 12:00AM EST
- Manuscript Accepted: 12 MAR 2009
- Manuscript Received: 23 SEP 2008
- NIH. Grant Numbers: R01-DK056084, F31-DK065312, P30-DK26743 (to the UCSF Liver Center)
- American Physiological Society–Porter Physiology Fellowship
- NIH/NCRR. Grant Number: CO6 RR018928 to Gladstone Institutes
- J. David Gladstone Institutes
Additional Supporting Information may be found in the online version of this article.
|HEP_22980_sm_SupFig1.tif||5681K||Supporting Figure 1. (A) AMPK signaling in livers Dgat1 and Dgat1 mice fed high-fat diet. Livers were isolated after mice were fasted for 4 hours. AMPK signaling was quantified by western blot analysis using antibodies specific for the phosphorylated forms of AMPK(Thr172) and ACC(S79) (cell signaling). Antibodies specific for LRP (gift from Joachim Herz) and total AMPK were used as loading controls (age 12 weeks, 3 week high-fat diet). (B) Targeting strategy used to generate Dgat1flox/flox mice shows the targeting vector, wild-type allele, Dgat1flox allele, and Dgat1flox allele after Cre-induced recombination. (C) Southern probe on exons 14-17 indicate genotype of wild-type and Dgat1?/flox mice.|
|HEP_22980_sm_SupFig2.tif||5681K||Supporting Figure 2. (A) Serum triglycerides and (B) free fatty acid levels in fasted Dgat1 and Dgat1 mice (Age 12 weeks, n = 5 per group).|
|HEP_22980_sm_SupFig3.tif||5681K||Supporting Figure 3. (A) Real-time PCR analysis of Fasn, Dgat1 and Dgat2 in livers of mice administered with T0901317 (n=5). (B) Liver mass and (C) hepatic TG in mice administered T0901317. ASO was administered twice per week i.p. at 50 mg/kg. After 4 weeks of treatment with ASO's, mice were treated with 50 mg/kg of T0901317 or sham for 11 days (For all, age 15-16 weeks, n = 5 per group).|
|HEP_22980_sm_SupTab1.doc||58K||Supplemental Table 1|
|HEP_22980_sm_SupplTab2.pdf||55K||Supplemental Table 2 Primer sequence used for real-time PCR.|
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