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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
HEP_22982_sm_SupDocument.doc108KSupplementary Data
HEP_22982_sm_SupFig1.tif3161KSupporting Figure 1 Northern blot of MiRNA levels during five days of (A) hepatocytic differentiation (DMSO) and (B) bile ductular differentiation in Matrigel. (L), liver; (U6), U6 small nuclear RNA loading control. MiRNAs as labeled. (C) QRT-PCR confirmation of miR-23b and miR-27b up regulation during DMSO induced differentiation of HBC-3 cells.
HEP_22982_sm_SupFig2.tif2292KSupporting Figure 2 (A) Mapping of two candidate transcription start sites in the RIKEN 201011i01Rik locus upstream from the miR23b cluster on mouse chromosome 13. B. RTPCR detection of a transcript from the miR-23b locus in undifferentiated HBC-3 cell RNA. (C) Quantitative plot of the expression of the RIKEN locus by cDNA microarray. (Diamonds)- DMSO treatment of HBC-3 cells. (Squares) Matrigel treatment of HBC-3 cells.
HEP_22982_sm_SupFig3.tif481KSupporting Figure 3 Mapping of transcription factor binding sites upstream of the miR-23b locus trancription start sites.
HEP_22982_sm_SupFig4.tif1011KSupporting Figure 4. qRT-PCR verifying knock down of miRNAs in HBC-3 cells transfected with miRidian Inhibitors.
HEP_22982_sm_SupTab1.doc170KSupporting Table 1. Summary of significantly expressed miRNAs detected by miRGEM hybridization (16) of small RNAs isolated from e 16.5, 17.5, P1 and adult mouse liver. Data sorted into families and clusters of miRNAs. The relative abundance (number in each box) is equal to the average of spot intensity (four spots/miRNA) for a miRNA divided by the average spot intensity for the entire set of expressed miRNAs. Red demarks above average expression. Green demarks below average expression. Yellow demarks equal to the average expression.
HEP_22982_sm_SupTab2.doc95KSupporting Table 2. List of all the candidate miR23b, 27b, and 24 target sites identified in the 3′UTR sequences of Smads 3, 4, and 5. Complementarity of the mature (guide) strand of miRNA to the mRNA target site are shown. -?G= the negative free energy of folding. Nucleotide numbers are the position in the 3′ UTR. Regions of sequence complementarity are shown as double line with non-comlementary nucleotides above or below the double line.
HEP_22982_sm_SupTab3.doc96KSupporting Table 3. Bioinformatic survey of candidate 3′ UTR target sites for miR-23b cluster miRNAs in genes of the TGFβ?BMP/Activin signal transduction networks C= conserved site, N = non-conserved site, Numbers= number of sites in the 3′ UTR.

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