Potential conflict of interest: Nothing to report.
Mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells during mouse liver development†
Article first published online: 6 APR 2009
Copyright © 2009 American Association for the Study of Liver Diseases
Volume 50, Issue 1, page 320, July 2009
How to Cite
Ramadori, G. and Mansuroglu, T. (2009), Mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells during mouse liver development. Hepatology, 50: 320. doi: 10.1002/hep.23003
- Issue published online: 23 JUN 2009
- Article first published online: 6 APR 2009
- Accepted manuscript online: 6 APR 2009 12:00AM EST
To the Editor:
Asahina and colleagues1 have to be congratulated for the very interesting data and for the enormous amount of work they spent to strengthen their conclusions. The authors demonstrate that fetal mouse liver contains at least three populations of mesenchymal cells.
- AThe mesothelial cells which are desmin-positive but negative for smooth muscle actin-alpha (SMAα).
- BThe perivascular mesenchymal cells which are desmin and SMA-positive with single desmin-positive SMA-negative cells.
- CThe parenchymal mesenchymal cells (hepatic stellate cells [HSCs]) which are desmin-positive and SMAα-negative with single exceptions (SMAα-positive and desmin-positive).
The latter cells were enriched by fluorescence-activated cell sorting for HSCs. Real-time polymerase chain reaction analysis and complementary DNA microarray analysis using total RNA from enriched cells confirmed the expression of the expected genes. Authors selected activated leukocyte cell adhesion molecule (ALCAM)-positive mesothelial cells and cultured them under different conditions. All cells became SMAα-positive (myofibroblastic phenotype). ALCAM-expressing cells were cultured on collagen in the presence of retinol and palmitic acid. These cells showed the capacity to develop fat droplets, suggesting that they could acquire the HSC phenotype. Because we could make an observation similar to that mentioned under cell population (B) using rat fetal livers,2 we would like to ask following questions:
- 1Could the single desmin-positive and SMAα-positive cells of the parenchyma (in Fig. 2E,F of Asahina et al.) represent the cells of the arterial capillary of the sinusoids?
- 2Did the enriched HSC population contain SMAα RNA?
- 3Is it possible that the desmin-positive, SMAα-negative cells (HSCs) do not survive in culture?
- 4If they survive (ALCAM-high) acquiring the myofibroblastic phenotype, does the SMAα gene expression decrease when they are cultured on collagen in the presence of retinol and palmitic acid, and does ALCAM remain positive (immunocytology)?
- 5Is it possible to make similar experiments using enriched HSCs?
- 1Mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells during mouse liver development. HEPATOLOGY 2009; 49: 998–1011., , , , , , et al.
- 2Hepatoblast- and mesenchymal cell-specific gene-expression in fetal rat liver and in cultured fetal rat liver cells. Histochem Cell Biol 2009. In press., , , , .
Giuliano Ramadori M.D.*, Tümen Mansuroglu M.D.*, * Department of Internal Medicine, Section of Gastroenterology and Endocrinology, Georg-August-University Göttingen, Göttingen, Germany.