Up-regulated microRNA-143 transcribed by nuclear factor kappa B enhances hepatocarcinoma metastasis by repressing fibronectin expression†
Article first published online: 6 APR 2009
Copyright © 2009 American Association for the Study of Liver Diseases
Volume 50, Issue 2, pages 490–499, August 2009
How to Cite
Zhang, X., Liu, S., Hu, T., Liu, S., He, Y. and Sun, S. (2009), Up-regulated microRNA-143 transcribed by nuclear factor kappa B enhances hepatocarcinoma metastasis by repressing fibronectin expression. Hepatology, 50: 490–499. doi: 10.1002/hep.23008
Potential conflicts of interest: none.
- Issue published online: 29 JUL 2009
- Article first published online: 6 APR 2009
- Accepted manuscript online: 6 APR 2009 12:00AM EST
- Manuscript Accepted: 31 MAR 2009
- Manuscript Received: 21 JAN 2009
- National Natural Science Foundation of China. Grant Numbers: 30530660, 30671829, 30671920
Additional Supporting Information may be found in the online version of this article.
|HEP_23008_sm_SupFig1.tif||1098K||Supplementary Figure 1. The levels of ectopic HBx in livers of transgenic mice and human HBV-HCC. (A) RT-PCR of hbx in 22-month-old p21-HBx transgenic mice and human HBV-HCC liver tissues. (B) Western blot of HBx in the livers of 22-month-old p21-HBx transgenic mice and human HBV-HCC using primary antibodies specific for HBx (Abcam), or β-actin (Cell Signaling) and goat anti-mouse secondary antibody.|
|HEP_23008_sm_SupFig2.tif||3130K||Supplementary Figure 2. Correlation of 11 miRNAs levels between miRNA microarray and real-time PCR analyses for HBx transgenic mice. Left panel: down-regulation of miRNAs assessed by microarray and real-time PCR in HBx transgenic mice compared to wild-type mice. Data are shown as means±S.D, based on at least three independent experiments. Middle panel: up-regulation of miRNAs, assessed by microarray and real-time PCR in HBx transgenic mice as compared to wild-type mice. Data are shown as means±S.D, based on at least three independent experiments. Right panel: the 11 miRNAs levels were assessed by coefficient analysis. The same samples were independently assessed by microarray and real-time PCR, and the results in both methodologies demonstrated very high internal consistency with a correlation coefficient (R2) of 0.973, and p value < 0.01 (Pearson).|
|HEP_23008_sm_SupTab1.doc||59K||Supplementary Table 1. Clinical background of HCC patients|
|HEP_23008_sm_SupTab2.doc||583K||Supplementary Table 2. Candidate genes of FNDC3B, predicted by TargetScan (Release 4.2: April 2008, http://www.targetscan.org/).|
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