Additional Supporting Information may be found in the online version of this article.

HEP_23048_sm_SupFig1.tif14457KSupporting Figure 1 Anti-Myc immunostaining detects the Myc-tagged transgenic Notch2ICD protein but no endogenous mouse c-Myc. Liver cryosections from embryonic (E16.5, E18.5) N2ICD/AlbCre and control mice were immunostained using an anti-Myc antibody raised against the human c-Myc epitope. Staining for DAPI and Myc shows that the anti-Myc antibody specifically detects Myc-tagged Notch2ICD in N2ICD/AlbCre mice, but no endogenous c-Myc protein in control mice. Nuclear colocalization of DAPI- and Myc- immunostaining shows that the transgenic Notch2ICD protein efficiently translocates into the nucleus. Scale bar 50μm.
HEP_23048_sm_SupFig2.tif8867KSupporting Figure 2 N2ICD/AlbCre mice show postnatal compensatory hepatocyte proliferation. (A) Costaining for DAPI, DBA and Ki67 in E18.5, P4 and P90 liver sections from control and N2ICD/AlbCre mice (n=3 per genotype) show proliferation in hepatocytes but not in DBA+ BECs. While hepatocyte proliferation is increased in N2ICD/AlbCre mice at P4 and P90 compared to control mice, no difference was observed at 18.5. (B) Quantification of the immunostained Ki67+ cells in percent of DAPI for control N2ICD/AlbCre mice are shown in a bar diagram. (C) Quantification of the liver weight as a percentage of body weight shows an increased liver weight in P4, P10 and P90 N2ICD/AlbCre mice compared to control mice. Bar diagrams (B, C) show mean ± SEM. P*≤ 0.05, P**≤ 0.01, P***≤ 0.001.
HEP_23048_sm_SupFig3.tif1420KSupporting Figure 3 Adult N2ICD/AlbCre livers exhibit normal values for bilirubin, alanine aminotransferase (ALAT) and alkaline phosphatase (AP). Blood plasma from adult (P90) control and N2ICD/AlbCre mice (n=5) was analyzed for changes in bilirubin (A), ALAT (B) and AP (C). 1 out of 5 N2ICD/AlbCre mice showed an increased bilirubin value.

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