SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
HEP_23083_sm_SupFig1.tif22655KSupporting Figure 1: Localization of BSEP in the patient's transplanted livers. Biopsies from the patient's livers 2 (A) and 3 (B) were stained for BSEP in red and MRP2 in green. Both proteins co-localized in the canalicular membrane resulting in a yellow colouring. Bars = 10 μm.
HEP_23083_sm_SupFig2.tif22655KSupporting Figure 2: Immunohistological localization of BSEP and the tight junction protein ZO-1 in the patient's liver. BSEP and ZO-1 were co-stained in biopsies from the patient's liver (A, B) and a control liver. The tight junction complex delineates the canalicular from the basolateral membrane of hepatocytes. In control livers (C) the BSEP staining (red) is predominantly detected between the linear ZO-1 staining (green) of the tight junction complex. The same staining pattern was observed in biopsies from the patient's liver (A, B), indicating that BSEP is mainly localized within the canalicular membrane. Bars = 10 μm.
HEP_23083_sm_SupFig3.tif22655KSupporting Figure 3: Effects of mutations on the expression and localization of BSEP. Human BSEP was cloned and tagged to the yellow fluorescent protein (YFP) and expressed in HEK293 cells. (A) Wild-type BSEP-YFP is localized in the plasma membrane of transfected HEK293 cells. (B) Introduction of the V444A mutation did not alter the membrane localization of BSEPV444A-YFP. (C) The Y818F missense mutation did not affect the localization of BSEPY818F-YFP. (D) The combination of V444A and Y818F had no effect on the membrane localization of mutated BSEP. (E) Introduction of the G982R mutation caused retention of BSEPG982R-YFP in the endoplasmic reticulum. (F) Combination of V444A with G982R led to the localization of the mutated protein in the endoplasmic reticulum. (G) The combination of Y818F with G982R caused localization of the mutated protein in the endoplasmic reticulum. (H) Introduction of the patient's mutations (V444A, Y818F, G982R) caused the complete absence of the mutated protein. Bars=10 μm.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.