Role of microRNA-155 at early stages of hepatocarcinogenesis induced by choline-deficient and amino acid–defined diet in C57BL/6 mice†
Article first published online: 29 MAY 2009
Copyright © 2009 American Association for the Study of Liver Diseases
Volume 50, Issue 4, pages 1152–1161, October 2009
How to Cite
Wang, B., Majumder, S., Nuovo, G., Kutay, H., Volinia, S., Patel, T., Schmittgen, T. D., Croce, C., Ghoshal, K. and Jacob, S. T. (2009), Role of microRNA-155 at early stages of hepatocarcinogenesis induced by choline-deficient and amino acid–defined diet in C57BL/6 mice. Hepatology, 50: 1152–1161. doi: 10.1002/hep.23100
Potential conflict of interest: Nothing to report.
- Issue published online: 28 SEP 2009
- Article first published online: 29 MAY 2009
- Accepted manuscript online: 29 MAY 2009 12:00AM EST
- Manuscript Accepted: 17 MAY 2009
- Manuscript Received: 20 MAR 2009
- National Institutes of Health. Grant Numbers: CA086978, CA101956
Additional Supporting Information may be found in the online version of this article.
|HEP_23100_sm_SupDoc.doc||187K||Supplemental Materials and Methods|
|HEP_23100_sm_SupFig1.tif||8760K||Figure S1. Pathological changes associated with multistage hepatocarcinogenesis in mice fed CDAA diet. Arrows indicate liver tumors.|
|HEP_23100_sm_SupFig2.tif||5678K||Figure S2. A and B. Northern blot analysis of miR-21 and miR-122 in the liver. Total RNA (30 μg) was separated in 15% acrylamide gel denaturing (8M urea) gel, transferred to nylon membrane followed by hybridization to 32P-labeled anti-sense miR-21/122 probes. The signal was developed by autoradiography and quantified using Kodak Imaging software.|
|HEP_23100_sm_SupFig3.tif||4395K||Figure S3. Immunohistochemical analysis of C/EBPβ in a HCC and benign liver tissues. Formalin-fixed HCC sections were subjected to immunohistochemistry analysis with anti-C/EBPβ antibody using Ventana Ultraview Universal Red system. C/EBPβ signal is red (green arrow) and the cell body is light blue.|
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