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HEP_23168_sm_supptext.doc36KSupplementary Materials
HEP_23168_sm_suppfig1.tif2584KSupplementary Figure 1. CD11b negatively regulates mature NK cell function. (A-B) Statistics of granzyme B production of sorted splenic CD11bhigh NK cells, derived from C57BL/6 mice, stimulated with or without Poly I:C (20μg/ml) in the presence or absence of neutralizing anti-CD11b or isotype antibody in vitro. Percentage of granzyme B+ NK cells in total NK cells and granzyme B MFI were shown. **p<0.01 vs. isotype group. (C-D) CD11b−/− (C57BL/6 background) NK cells produced more granzyme B than C57BL/6 (WT) NK cells stimulated with or without Poly I:C (20μg/ml) in vitro. Percentage of granzyme B+ NK cells in total NK cells and granzyme B MFI were shown. **p<0.01 vs. that on (WT) C57BL/6 NK cells with the same stimulation. All data are representative of three independent experiments.
HEP_23168_sm_suppfig2.tif680KSupplementary Figure 2. CD11b deficiency has no effect on perforin expression of NK cells stimulated by Poly I:C. C57BL/6 (WT) splenic NK cells and CD11b−/− splenic NK cells were stimulated with or without Poly I:C (20μg/ml) for 6-8 h in vitro, and intracellular perforin expression was analyzed by FACS as shown. The data are representative of three independent experiments.
HEP_23168_sm_suppfig3.tif1320KSupplementary Figure 3. No involvement of NKT cells in the Poly I:C-mediated acute hepatitis. WT BALB/c mice (n=6) and CD1d deficient (CD1d−/−, BALB/c background) mice (n=6) were injected with Poly I:C (20μg/g body wt), and then serum ALT (A) and AST (B) levels were assayed 20 h later as described in Fig. 6. **p<0.01. All data are representative of three independent experiments.
HEP_23168_sm_suppfig4.tif3442KSupplementary Figure 4. Poly I:C induces significant increase of CD11b abundance on NK cells from adult mice. C57BL/6 mice of 8-12 weeks age were injected with PBS or Poly I:C (20μg/ml) for 20 h, and then the CD11b expression on splenic NK cells was analyzed as shown. **p<0.01. The experiments were performed for five times with similar results.
HEP_23168_sm_suppfig5.tif1368KSupplementary Figure 5. No involvement of liver macrophages (Kupffer cells) in the attenuation of PolyI:C-mediated liver injury by CD11b. GdCl3, a kind of Kupffer cell specific toxin, was intravenously injected at dosage of 20mg/kg into C57BL/6 mice (n=6) and CD11b−/− mice (n=6). Twenty four hours later, macrophages in the liver would be specifically dysfunctional, then Poly I:C (20μg/ml) was injected intraperitoneally, and the serum ALT and AST were assayed 20 h later as described in Fig. 6. **p<0.01. The data are representative of three independent experiments.

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