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HEP_23173_sm_SuppFig1.tif288KSupporting Fig. 1. Elevated mRNA of B7-H1 and B7-DC in chronically inflamed livers. RNA was extracted from formalin-fixed paraffin embedded liver needle biopsies. Quantitative real-time PCR was performed utilizing Applied Biosystems TaqMan probes. The expression of B7-H1 and B7-DC was normalized against 18S. The expression of mRNA was increased in livers from patients with chronic necroinflammatory disease. (Normal, n=7; HCV, AIH, NAFLD n=5 per group).
HEP_23173_sm_SuppFig2.tif1249KSupplemental Figure 2. Up-regulation of B7-H1 and HLA A, B, C on cultured Huh 7.5 cells after IFN-γ treatment. Huh 7.5 cells were cultured with or without interferon-?. Flow cytometry was performed on these cells. Expression of HLA A, B, C was demonstrated on these cells, which was upregulated following exposure to interferon-γ. There was no expression of B7-H1 on Huh 7.5 at time zero, but there was expression of B7-H1 after interferon-γ.
HEP_23173_sm_SuppDoc.doc32KSupporting Methods and Materials

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