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These authors contributed equally to this study.
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Liver Injury/Regeneration
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In vivo phosphoenolpyruvate carboxykinase promoter mapping identifies disrupted hormonal synergism as a target of inflammation during sepsis in mice†
Article first published online: 4 AUG 2009
DOI: 10.1002/hep.23194
Copyright © 2009 American Association for the Study of Liver Diseases
Additional Information
How to Cite
Chichelnitskiy, E., Vegiopoulos, A., Diaz, M. B., Ziegler, A., Kleiman, A., Rauch, A., Tuckermann, J. and Herzig, S. (2009), In vivo phosphoenolpyruvate carboxykinase promoter mapping identifies disrupted hormonal synergism as a target of inflammation during sepsis in mice. Hepatology, 50: 1963–1971. doi: 10.1002/hep.23194
- †
Potential conflict of interest: Nothing to report.
Publication History
- Issue published online: 20 NOV 2009
- Article first published online: 4 AUG 2009
- Accepted manuscript online: 4 AUG 2009 12:00AM EST
- Manuscript Accepted: 18 JUL 2009
- Manuscript Received: 5 MAR 2009
Funded by
- Deutsche Forschungsgemeinschaft. Grant Numbers: He3260/2-1, Tu220/3
- European Foundation for the Study of Diabetes
- Marie Curie Excellence Grant (European Commission)
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Additional Supporting Information may be found in the online version of this article.
| Filename | Format | Size | Description |
|---|---|---|---|
| HEP_23194_sm_SupFig1.tif | 907K | Supporting Figure 1. A, Relative luciferase activity in H4IIE hepatocytes infected with an adenovirus lacking all PEPCK 5′-flanking regions. Cells were treated with forskolin (FSK, 10 μM) and/or dexamethasone (DEX, 10 nM) as indicated for 24 h before harvesting the cells (means ± SEM, n=9). B, Blood glucose levels of C57Bl6 mice infected with adenoviruses carrying distinct PEPCK promoter luciferase fragments at day 5 after injection as indicated. Mice were either fasted for 12 h or fasted for 12 h and refed for 6 h (means ± SEM, n=9). C, Serum levels of interferon (Ifn)γ, interleukin (IL)1β, IL6, and tumor necrosis factor (TNF)α in C57Bl6 mice treated with lipopolysaccharide (LPS; 20 μg/g body weight) for 8 h (means ± SEM, n=9). D, Serum total ketone body levels in same animals as in C (means ± SEM, n=9). *, p < 0.05. | |
| HEP_23194_sm_SupFig2.tif | 958K | Supporting Figure 2. A-D, Quantitative PCR analysis of hepatic PEPCK mRNA (left panels) and blood glucose (right panels) levels in C57Bl6 mice infected with adenoviruses carrying 1330 (A), 490 (B-D) or 355 (D) bp of wild-type PEPCK promoter luciferase fragments or 490 bp PEPCK promoter fragments harboring point mutations within the PEPCK GRE (B) or CRE (C) at day 5 after injection. Mice were fasted overnight and injected with LPS (20 μg/g body weight) for 8 h (means ± SEM, n=9). *, p ≤ 0.05. | |
| HEP_23194_sm_SupFig3.tif | 218K | Supporting Figure 3. A, Transient transfection assay of H4IIE hepatocytes transfected with luciferase reporter constructs carrying 1000 bp of the PGC-1α 5′-flanking region (PGC1αLuc) or 3 copies of an isolated nuclear factor κ B (NFκB)/RelA/p65 response element (3xNFκB). Cells were treated with LPS-conditioned macrophage medium (RAW) for 24 h as indicated (means ± SEM, n=9). *, p ≤ 0.05. |
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