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HEP_23248_sm_SuppFig.tif878KAmino acid concentrations similar to those in plasma of patients with advanced cirrhosis impaired maturation of myeloid DCs. We cultured monocytes under CCM with GM-CSF and IL-4 for 6 days and exposed them to poly I:C for an additional 24 hours. Cells were harvested and stained with different mAbs, and analyzed using flow cytometry. The percentages indicate the proportion of cells adopting the DC immunophenotype (BDCA1+/CD86+ or CD83+/CD98+). Results are representative of three experiments from three different donors.

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