These authors contributed equally to this study.
Inhibition of poly adenosine diphosphate-ribose polymerase decreases hepatocellular carcinoma growth by modulation of tumor-related gene expression†
Article first published online: 25 AUG 2009
Copyright © 2009 American Association for the Study of Liver Diseases
Volume 51, Issue 1, pages 255–266, January 2010
How to Cite
Quiles-Perez, R., Muñoz-Gámez, J. A., Ruiz-Extremera, Á., O'Valle, F., Sanjuán-Nuñez, L., Martín-Álvarez, A. B., Martín-Oliva, D., Caballero, T., Muñoz de Rueda, P., León, J., Gonzalez, R., Muntané, J., Oliver, F. J. and Salmerón, J. (2010), Inhibition of poly adenosine diphosphate-ribose polymerase decreases hepatocellular carcinoma growth by modulation of tumor-related gene expression. Hepatology, 51: 255–266. doi: 10.1002/hep.23249
Potential conflict of interest: Nothing to report.
- Issue published online: 23 DEC 2009
- Article first published online: 25 AUG 2009
- Accepted manuscript online: 25 AUG 2009 12:00AM EST
- Manuscript Accepted: 13 AUG 2009
- Manuscript Received: 27 APR 2009
- Ciberehd (Ciberehd is funded by the Instituto de Salud Carlos III)
- Junta de Andalucía. Grant Number: 264/03
- Ministerio de Educación y Ciencia. Grant Number: SAF2006-01094
- Fundación LA CAIXA. Grant Number: BM06-219-0
Additional Supporting Information may be found in the online version of this article.
|HEP_23249_sm_SupDoc.doc||44K||SUPPORTING MATERIAL AND METHODS|
|HEP_23249_sm_SupFig1.tif||5433K||Figure S1. Characterisation of PARP-1 silenced in HCC (PLC-PRF-5 C6 clone) and non-tumoural (WRL68 C1 clone) transtected with pGeneClip-U1-siP912 vector, analyzed for PARP-1 expression: fig A, PARP-1 expression determinated by means of qRT-PCR; figure B, Western Blot against PARP-1 and figure C, PARP-1 immunofluorescence.|
|HEP_23249_sm_SupFig2.tif||348K||Figure S2. PARP activity and its inhibition by DPQ in HCC xenografts. Error bars represent SE of the mean (SEM).|
|HEP_23249_sm_SupFig3.tif||461K||Figure S3. PARP inhibition decreases the formation of vessel-like structures and the DPQ did not reveal any cytotoxic effect at the different concentrations (PI staining and flow-cytometry analysis).|
|HEP_23249_sm_SupFig4.tif||469K||Figure S4. PARP-1, EGFR and ENG (END) expression in various PLC-PRF-5 clones. The reduction of gene expression was dependent of the intensity of PARP-1 blockage, confirming the role of PARP-1 in the expression of these genes.|
|HEP_23249_sm_SupFig5A-C.tif||8390K||Figure S5. PARP-1 is over-expressed in human HCC in contrast to normal liver cells.|
|HEP_23249_sm_SupFig5D.tif||436K||Figure S5. PARP-1 is over-expressed in human HCC in contrast to normal liver cells.|
|HEP_23249_sm_SupTab1.doc||224K||Supplementary Table 1|
|HEP_23249_sm_SupTab2.doc||172K||Supplementary Table 2|
|HEP_23249_sm_SupTab3.doc||103K||Supplementary Table 3|
|HEP_23249_sm_SupTabs4and5.doc||38K||Table S4. Transaminase levels in xenograft model. Table S5. Transaminase levels in DEN model.|
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