Additional Supporting Information may be found in the online version of this article.

HEP_23249_sm_SupFig1.tif5433KFigure S1. Characterisation of PARP-1 silenced in HCC (PLC-PRF-5 C6 clone) and non-tumoural (WRL68 C1 clone) transtected with pGeneClip-U1-siP912 vector, analyzed for PARP-1 expression: fig A, PARP-1 expression determinated by means of qRT-PCR; figure B, Western Blot against PARP-1 and figure C, PARP-1 immunofluorescence.
HEP_23249_sm_SupFig2.tif348KFigure S2. PARP activity and its inhibition by DPQ in HCC xenografts. Error bars represent SE of the mean (SEM).
HEP_23249_sm_SupFig3.tif461KFigure S3. PARP inhibition decreases the formation of vessel-like structures and the DPQ did not reveal any cytotoxic effect at the different concentrations (PI staining and flow-cytometry analysis).
HEP_23249_sm_SupFig4.tif469KFigure S4. PARP-1, EGFR and ENG (END) expression in various PLC-PRF-5 clones. The reduction of gene expression was dependent of the intensity of PARP-1 blockage, confirming the role of PARP-1 in the expression of these genes.
HEP_23249_sm_SupFig5A-C.tif8390KFigure S5. PARP-1 is over-expressed in human HCC in contrast to normal liver cells.
HEP_23249_sm_SupFig5D.tif436KFigure S5. PARP-1 is over-expressed in human HCC in contrast to normal liver cells.
HEP_23249_sm_SupTab1.doc224KSupplementary Table 1
HEP_23249_sm_SupTab2.doc172KSupplementary Table 2
HEP_23249_sm_SupTab3.doc103KSupplementary Table 3
HEP_23249_sm_SupTabs4and5.doc38KTable S4. Transaminase levels in xenograft model. Table S5. Transaminase levels in DEN model.

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