Additional Supporting Information may be found in the online version of this article.

HEP_23265_sm_SuppFig1.tif10451KSupporting Fig. 1. Histology of liver tissue from Ostα+/+ and Ostα−/− mice. Hematoxylin and eosin staining demonstrates that livers from both Ostα+/+ and Ostα−/− BDL mice had increased numbers of infarcts, although blinded analysis did not find a significant difference between the groups.
HEP_23265_sm_SuppFig2.tif357KSupporting Fig. 2. Hepatic bile flow rates, and biliary excretion of total bile acids, cholesterol, and phospholipids in Ostα+/+ and Ostα−/− mice. Bile samples were collected from the common bile duct at 30 minutes intervals for 90 minutes in 5 male Ostα+/+ and 4 male Ostα−/− untreated mice that had been fed ad libitum. These animals had not undergone any prior treatment or surgical manipulations. Bile volumes were determined gravimetrically, assuming a density of 1.0, and bile flow is expressed as μl/min.g liver. Biliary total bile acids, cholesterol, and phospholipids are expressed both as excretion rates per gram of liver (panel B, D, and F, respectively), and as concentrations (panel C, E, and G, respectively). Values are means ± SD. *Significantly different from Ostα+/+ mice, P < 0.05.
HEP_23265_sm_SuppTab.doc22KTable S1: Antibodies used in this study
HEP_23265_sm_SuppDoc.doc28KSupporting Material

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