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HEP_23267_sm_supptext.doc34KSupplementary Materials and Methods
HEP_23267_sm_suppfig1.tif22105KSupplemental Figure 1: Histological assessment of liver injury (hematoxilin & eosin, H&E) in control animals or mice treated with 300 mg/kg acetaminophen (APAP) for 6 h. Some of the animals received additionally 10 ml/kg saline, 0.65 mmol/kg GSH or 0.65 mmol/kg N-acetylcysteine (NAC) iv 1.5 h after APAP. The representative pictures show extensive centrilobular necrosis as indicated by the loss of basophilic staining, vacuolization, cell swelling and karyolysis in APAP-treated animals. Both, GSH and NAC treatment improved the area of necrosis with GSH being more effective than NAC. (x200 for all panels)
HEP_23267_sm_suppfig2.tif11749KSupplemental Figure 2: Concentrations of 13C-labelled [4,5-13C]glutamate and the Krebs cycle intermediate [2,3-13C]succinate (nmol/g wet weight), as calculated from their resonances in 1H- and 13C-NMR spectra of liver extracts. The mice were treated with 300 mg/kg APAP and some subsequently received additionally 10 ml/kg saline, 0.65 mmol/kg N-acetylcysteine (l-NAC), 1.95 mmol/kg NAC (h-NAC), a mixture of 3 amino acids (0.65 mmol/kg of glycine, glutamic acid and NAC) (3AS) or a mixture of 2 amino acids (0.98 mmol/kg glycine and glutamic acid) (2AS) iv 1.5 h after APAP. Data represent means ± SE of n = 5 animals per group. *P<0.05 (compared to controls)

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