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HEP_23273_sm_SupFig1.tif590KFigure 1. The percentages of CD4 T-cell subsets in various cohorts of subjects. (A) Pooled data indicate the percentages of Treg, Th1 and Th2 subsets in HC, CHB and ACLF groups, respectively. (B) Pooled data indicate the percentages of FoxP3+IL-17+, IFN-r+IL-17+ and IL-4+IL-17+ CD4 T cells in HC, CHB and ACLF groups, respectively. Each dot represents one individual. Horizontal bars represent the median values of indicated index. *P <0.05 and **P <0.01 are shown. HC, healthy controls; CHB, chronic hepatitis B; ACLF, acute-on-chronic liver failure.
HEP_23273_sm_SupFig2.tif297KFigure 2. Peripheral Th17 cells have no capacity to produce IL-22. (A) Representative dot plots of IL-17 and IL-22 co-expression in peripheral CD4 T cells of HC subjects, CHB and ACLF patients. The values in the quadrants indicate the percentage of each CD4 T-cell subset. (B) Pooled data indicate the percentages of IL-22-producing CD4 T cells in HC, CHB and ACLF groups, respectively. (C) Pooled data indicate the percentages of IL-22-producing Th17 cells in HC, CHB and ACLF groups, respectively. (B and C) The bars represent means and standard deviations.
HEP_23273_sm_SupFig3.tif244KFigure 3. IL-17 in vitro down-regulates IL-17R expression on monocytes and mDCs. Representative histogram indicates the expression of IL-17R on monocytes and mDCs from an HC subject. The isolated mDCs and monocytes were incubated with medium only or in the presence of IL-17 (1 ng/mL) in 96-well plates for 24 h. The stimulated cells were harvested at 6h, 12h and 24h, respectively, and IL-17R expression was analyzed using flow cytometry. Cells were gated on CD14+ cells and Lin1-CD11c+HLA-DR+ cells. Green histograms, IL-17R expression; Red histograms, isotype controls. The values in histograms represent mean fluorescence intensity of IL-17R expression. Data shown are representative of three independently performed experiments.

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