These authors contributed equally to this work.
Interleukin-17–producing CD4+ T cells increase with severity of liver damage in patients with chronic hepatitis B†
Version of Record online: 9 SEP 2009
Copyright © 2009 American Association for the Study of Liver Diseases
Volume 51, Issue 1, pages 81–91, January 2010
How to Cite
Zhang, J.-Y., Zhang, Z., Lin, F., Zou, Z.-S., Xu, R.-N., Jin, L., Fu, J.-L., Shi, F., Shi, M., Wang, H.-F. and Wang, F.-S. (2010), Interleukin-17–producing CD4+ T cells increase with severity of liver damage in patients with chronic hepatitis B. Hepatology, 51: 81–91. doi: 10.1002/hep.23273
Potential conflict of interest: Nothing to report.
- Issue online: 23 DEC 2009
- Version of Record online: 9 SEP 2009
- Accepted manuscript online: 9 SEP 2009 12:00AM EST
- Manuscript Accepted: 30 AUG 2009
- Manuscript Received: 21 APR 2009
- National Grand Program on Key Infectious Disease. Grant Numbers: 2009ZX10004-309, 2008ZX10002-007, 2008ZX10002-005-6
- National Key Basic Research Program of China. Grant Number: 2007CB512805
- Key Program of National Natural Science Foundation of China. Grant Number: 30730088
- National Laboratory of Biomacromolecules
- Institute of Biophysics
- China Academy of Sciences
Additional Supporting Information may be found in the online version of this article.
|HEP_23273_sm_SupFig1.tif||590K||Figure 1. The percentages of CD4 T-cell subsets in various cohorts of subjects. (A) Pooled data indicate the percentages of Treg, Th1 and Th2 subsets in HC, CHB and ACLF groups, respectively. (B) Pooled data indicate the percentages of FoxP3+IL-17+, IFN-r+IL-17+ and IL-4+IL-17+ CD4 T cells in HC, CHB and ACLF groups, respectively. Each dot represents one individual. Horizontal bars represent the median values of indicated index. *P <0.05 and **P <0.01 are shown. HC, healthy controls; CHB, chronic hepatitis B; ACLF, acute-on-chronic liver failure.|
|HEP_23273_sm_SupFig2.tif||297K||Figure 2. Peripheral Th17 cells have no capacity to produce IL-22. (A) Representative dot plots of IL-17 and IL-22 co-expression in peripheral CD4 T cells of HC subjects, CHB and ACLF patients. The values in the quadrants indicate the percentage of each CD4 T-cell subset. (B) Pooled data indicate the percentages of IL-22-producing CD4 T cells in HC, CHB and ACLF groups, respectively. (C) Pooled data indicate the percentages of IL-22-producing Th17 cells in HC, CHB and ACLF groups, respectively. (B and C) The bars represent means and standard deviations.|
|HEP_23273_sm_SupFig3.tif||244K||Figure 3. IL-17 in vitro down-regulates IL-17R expression on monocytes and mDCs. Representative histogram indicates the expression of IL-17R on monocytes and mDCs from an HC subject. The isolated mDCs and monocytes were incubated with medium only or in the presence of IL-17 (1 ng/mL) in 96-well plates for 24 h. The stimulated cells were harvested at 6h, 12h and 24h, respectively, and IL-17R expression was analyzed using flow cytometry. Cells were gated on CD14+ cells and Lin1-CD11c+HLA-DR+ cells. Green histograms, IL-17R expression; Red histograms, isotype controls. The values in histograms represent mean fluorescence intensity of IL-17R expression. Data shown are representative of three independently performed experiments.|
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