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Additional Supporting Information may be found in the online version of this article.

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HEP_23385_sm_SupportingFigure1.tif2124KSupporting Fig. 1. Deficiency of c-Rel has no effect on the extent of chronic liver damage. Serum markers for liver damage (ALT and AST) in mice treated with CCl4 for 12 wks. Serum was collected on days 1, 3, 7 and 10 after the final injection. Data are mean +/− SEM, n=5-6. ALT/AST values between c-rel−/− and Wt mice are not statistically significant (ns).
HEP_23385_sm_SupportingFigure2.tif8503KSupporting Fig. 2. Deficiency of c-Rel does not influence macrophage levels. Representative images and manual counts of CD68 immunostained liver sections to detect macrophages from mice treated with CCl4 twice weekly for 12 wks (A+B). Bar = 50μm. Manual count of F4/80 immunostained liver sections to detect macrophages from mice treated with a single injection of CCl4 (Abcam, 1:100, mouse monoclonal). Macrophage levels between c-rel−/− and Wt livers are not statistically significant (ns) at each time point analysed in the chronic and acute CCl4 model.
HEP_23385_sm_SupportingFigure3.tif2125KSupporting Fig. 3. Deficiency of c-Rel enhances the extent of acute liver damage. Serum markers for liver damage (ALT and AST) in mice treated with a single injection of CCl4. Serum was collected 4, 12, 24 and 48 hrs later. Data are mean +/− SEM, n=6-8. Unpaired t-test ALT 24 hrs, P = 0.0167; AST levels are not statistically significant (ns).
HEP_23385_sm_SupportingFigure4.tif6371KSupporting Fig. 4. Liver to body weight ratios after partial hepatectomy. Data are mean +/− SEM, n=3-5. Liver to body weight ratios after PHx between c-rel−/− and Wt mice are not statistically significant (ns) at all analysed time points.
HEP_23385_sm_SupportingTable1.tif2124KSupporting Table 1. Summary of all antibodies used in Western blot analysis and immunohistochemistry.
HEP_23385_sm_SupportingTable2.tif2124KSupporting Table 2. Primer sequences for the different genes analysed in this study in SYBR green based real-time PCR.
HEP_23385_sm_SupportingDocument.doc36KSupporting Information

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