These authors contributed equally to the manuscript.
The c-Rel subunit of nuclear factor-κB regulates murine liver inflammation, wound-healing, and hepatocyte proliferation†
Article first published online: 19 OCT 2009
Copyright © 2009 American Association for the Study of Liver Diseases
Volume 51, Issue 3, pages 922–931, March 2010
How to Cite
Gieling, R. G., Elsharkawy, A. M., Caamaño, J. H., Cowie, D. E., Wright, M. C., Ebrahimkhani, M. R., Burt, A. D., Mann, J., Raychaudhuri, P., Liou, H.-C., Oakley, F. and Mann, D. A. (2010), The c-Rel subunit of nuclear factor-κB regulates murine liver inflammation, wound-healing, and hepatocyte proliferation. Hepatology, 51: 922–931. doi: 10.1002/hep.23385
Potential conflict of interest: Nothing to report.
- Issue published online: 2 MAR 2010
- Article first published online: 19 OCT 2009
- Accepted manuscript online: 19 OCT 2009 12:00AM EST
- Manuscript Accepted: 1 OCT 2009
- Manuscript Received: 15 APR 2009
- UK Medical Research Council. Grant Number: G0401643
- Wellcome Trust. Grant Number: BH081446
- British Liver Trust
Additional Supporting Information may be found in the online version of this article.
|HEP_23385_sm_SupportingFigure1.tif||2124K||Supporting Fig. 1. Deficiency of c-Rel has no effect on the extent of chronic liver damage. Serum markers for liver damage (ALT and AST) in mice treated with CCl4 for 12 wks. Serum was collected on days 1, 3, 7 and 10 after the final injection. Data are mean +/− SEM, n=5-6. ALT/AST values between c-rel−/− and Wt mice are not statistically significant (ns).|
|HEP_23385_sm_SupportingFigure2.tif||8503K||Supporting Fig. 2. Deficiency of c-Rel does not influence macrophage levels. Representative images and manual counts of CD68 immunostained liver sections to detect macrophages from mice treated with CCl4 twice weekly for 12 wks (A+B). Bar = 50μm. Manual count of F4/80 immunostained liver sections to detect macrophages from mice treated with a single injection of CCl4 (Abcam, 1:100, mouse monoclonal). Macrophage levels between c-rel−/− and Wt livers are not statistically significant (ns) at each time point analysed in the chronic and acute CCl4 model.|
|HEP_23385_sm_SupportingFigure3.tif||2125K||Supporting Fig. 3. Deficiency of c-Rel enhances the extent of acute liver damage. Serum markers for liver damage (ALT and AST) in mice treated with a single injection of CCl4. Serum was collected 4, 12, 24 and 48 hrs later. Data are mean +/− SEM, n=6-8. Unpaired t-test ALT 24 hrs, P = 0.0167; AST levels are not statistically significant (ns).|
|HEP_23385_sm_SupportingFigure4.tif||6371K||Supporting Fig. 4. Liver to body weight ratios after partial hepatectomy. Data are mean +/− SEM, n=3-5. Liver to body weight ratios after PHx between c-rel−/− and Wt mice are not statistically significant (ns) at all analysed time points.|
|HEP_23385_sm_SupportingTable1.tif||2124K||Supporting Table 1. Summary of all antibodies used in Western blot analysis and immunohistochemistry.|
|HEP_23385_sm_SupportingTable2.tif||2124K||Supporting Table 2. Primer sequences for the different genes analysed in this study in SYBR green based real-time PCR.|
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