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Additional Supporting Information may be found in the online version of this article.

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HEP_23427_sm_SupDocument.doc67KSupporting Materials
HEP_23427_sm_SupFig1.tif218KSupporting Fig. 1. siRNA knock-down of gene expression in primary Kupffer cells. Kupffer cells isolated from pair- and ethanol-fed rats were transfected or not with 2.0 μg target siRNA or scrambled siRNA. Efficiency of siRNA knock-down was determined by measuring A) IL-10 mRNA relative to 18S mRNA (values represent means ± SEM, n=3, +p<0.05 compared to non-transfected cells and cells transfected with scrambled siRNA) or B) HO-1 protein expression or C) STAT3 protein expression by Western blot. Hsc70 was used as a loading control. Representative Western blots are shown. D) Adiponectin receptor 1 (AdipoR1) and E) adiponectin receptor 2 (AdipoR2) mRNA relative to 18S mRNA (values represent means ± SEM, n=3, +p<0.05 compared to non-transfected cells and cells transfected with scrambled siRNA).

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