MicroRNA-30d promotes tumor invasion and metastasis by targeting Galphai2 in hepatocellular carcinoma†
Version of Record online: 30 NOV 2009
Copyright © 2009 American Association for the Study of Liver Diseases
Volume 51, Issue 3, pages 846–856, March 2010
How to Cite
Yao, J., Liang, L., Huang, S., Ding, J., Tan, N., Zhao, Y., Yan, M., Ge, C., Zhang, Z., Chen, T., Wan, D., Yao, M., Li, J., Gu, J. and He, X. (2010), MicroRNA-30d promotes tumor invasion and metastasis by targeting Galphai2 in hepatocellular carcinoma. Hepatology, 51: 846–856. doi: 10.1002/hep.23443
Potential conflict of interest: Nothing to report.
- Issue online: 2 MAR 2010
- Version of Record online: 30 NOV 2009
- Accepted manuscript online: 30 NOV 2009 12:00AM EST
- Manuscript Accepted: 26 OCT 2009
- Manuscript Received: 25 JUL 2009
- Science & Technology Commission of Shanghai Municipality. Grant Number: 07DJ14006
- Ministry of Health of China. Grant Number: 2008ZX10002-017
- Ministry of Human Resources and Social Security of China. Grant Number: 2007-170
- Shanghai Municipal Bureau of Public Health. Grant Number: 2007082
Additional Supporting Information may be found in the online version of this article.
|HEP_23443_sm_Suppfig1.tif||602K||Supplementary Fig. S1 Expression of mature miR-30d in various HCC cell lines The relative expression level of mature miR-30d in various liver cancer cell lines was determined by quantitative Real-time PCR. Data are normalized by U6 snRNA respectively.|
|HEP_23443_sm_Suppfig2.tif||2285K||Supplementary Fig. S2 Establishment of stable cell lines highly expressing miR-30d A. The relative expression level of mature miR-30d in Huh-7, SMMC-7721 or SK-HEP-1 cells infected with lentivirus expressing either miR-30d or mock vector. B. The mature miR-30d expression level in stable SMMC-7721 cells under the pressure of G418. Quantitative RT-PCR assay is used, data are normalized by U6 snRNA, and fold changes are indicated in the form of mean ± S.E.M.|
|HEP_23443_sm_Suppfig3.tif||756K||Supplementary Fig. S3 Over-expression of miR-30d has no obvious effects on HCC cell growth in vitro A. The CCK-8 assay of SK-HEP-1 cells infected with lentivirus expressing miR-30d or mock control. One thousand cells per well are seeded, and measured every day. Mean values are plotted as shown, and the bars indicate S.E.M in triplet. B. The colony formation assay of SMMC-7721 cells infected with lentivirus expressing miR-30d or mock control. Five hundred cells per well are seeded and cultivated for 2 weeks. After fixation and dying, colonies of miR-30d are counted and normalized by mock control. The bars represent S.E.M from three independent experiments.|
|HEP_23443_sm_Suppfig4.tif||3101K||Supplementary Fig. S4 Sixty-nine genes that were down-regulated by miR-30d in HCC stable cells A. The expression profiles of 69 down-regulated genes in two stable clones of SMMC-7721 cells expressing miR-30d. Green color represents the ratio of these down-regulated genes in SMMC-7721-30d/SMMC-7721-Mock. Sharper the color, lower the ratio. B. The name and expression information of these 69 genes are listed.|
|HEP_23443_sm_Suppfig5.tif||1209K||Supplementary Fig. S5 Expression of GNAI2 in various HCC cell lines The relative expression level of GNAI2 in various HCC cell lines was determined by quantitative Real-time PCR. Data are normalized by β-actin respectively, and the bars stand for S.E.M.|
|HEP_23443_sm_Suppfig6.tif||785K||Supplementary Fig. S6 GNAI2 interaction with miR-30 family A. Schema of predicted binding sites of miR-30 family within the 3′UTR of GNAI2 by three online prediction algorithms TargetScan, PicTar and MicroCosm Targets. B. Western blot assays of endogenous GNAI2 protein level in SK-HEP-1 cells after transfected with miR-30a, b, c, e or negative control.|
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