SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
HEP_23468_sm_Suppfig1.tif598KSupplementary figure S1. HCC sections of HBx transgenic mouse livers were stained for immunofluorescence to simultaneously detect PTTG1 (red) and HBx (green). Yellow color indicates overlap of proteins. Bar, 50 μm.
HEP_23468_sm_Suppfig2.tif183KSupplementary figure S2. (A) Chang liver, p34X, AML12 4p and 4pX cells were cultured with or without Dox for 24 h, and HBx and PTTG1 protein levels were analyzed by Western blot. Tubulin expression was assessed to ensure equal protein loading of all samples. (B) Flow cytometry analysis of cell cycle progression of p34X and 4pX cells 24 h after induction of HBx expression. Values represent the mean ± SD of three independent experiments performed at least in duplicate. (*p≤0,05 vs HBx non-expressing cells, Mann-Whitney U test).
HEP_23468_sm_Suppfig3.tif25KSupplementary figure S3. PTTG1 mRNA levels in 4pX cells in response to HBx expression were measured by quantitative RT-PCR. The results were normalized with histone H3 and represented as fold induction over control. Results are presented as the mean ± SD of three independent experiments.
HEP_23468_sm_Suppfig4.tif278KSupplementary figure S4. (A) Chang liver cells were treated with okadaic acid (OA) (1 μM), MG132 (10 μM) or both for 2 h after 48 h of doxycycline treatment. Lysates were subsequently analyzed by Western blot for the detection of PTTG1. Tubulin expression was assessed to ensure equal protein loading. (B) Hela cells were co-transfected with pCGN-HA-ubiquitin, pcDNA-PTTG1 and either pSV-HBx or pSV-hygro plasmids and treated for 4 h with MG132 (10 μM). Cell lysates were immunoprecipitated with anti-PTTG1 mAb and immunoblotted with anti-PTTG1 (bottom) and anti-HA (top) Abs. Representative results of at least two independent experiments are shown.
HEP_23468_sm_Suppfig5.tif135KSupplementary figure S5. Chang liver cells were treated for 2 h with okadaic acid (OA; 1μM) and/or MG132 (10μM) after 48 h of transfection with control or Cul1 siRNA. Lysates were subsequently analyzed by Western blot for the detection of PTTG1 and Cul1. Tubulin expression was assessed to ensure equal protein loading.
HEP_23468_sm_Supptext.doc79KSUPPLEMENTARY DATA

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.