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Lactoferrin protects against acetaminophen-induced liver injury in mice†
Article first published online: 30 NOV 2009
Copyright © 2009 American Association for the Study of Liver Diseases
Volume 51, Issue 3, pages 1007–1016, March 2010
How to Cite
Yin, H., Cheng, L., Holt, M., Hail, N., MacLaren, R. and Ju, C. (2010), Lactoferrin protects against acetaminophen-induced liver injury in mice. Hepatology, 51: 1007–1016. doi: 10.1002/hep.23476
Potential conflict of interest: Nothing to report.
- Issue published online: 2 MAR 2010
- Article first published online: 30 NOV 2009
- Accepted manuscript online: 30 NOV 2009 12:00AM EST
- Manuscript Accepted: 23 OCT 2009
- Manuscript Received: 12 MAY 2009
- U.S. National Institutes of Health. Grant Number: RO1 ES012914
- American College of Clinical Pharmacy Frontiers Career Development Research Award
Additional Supporting Information may be found in the online version of this article.
|HEP_23476_sm_SuppFig1.tif||1953K||Supporting Fig. 1. Human holo-Lac and non-holo-Lac protected against AILI in mice. Male Balb/cJ mice were i.v. injected with 50 mg/kg of human holo-Lac (APAP/Holo-Lac), or non-holo-Lac (APAP/Non-holo-Lac), or PBS (APAP/PBS) at 1 and 4 h after APAP challenge (300 mg/kg). Serum ALT levels were determined at 8 and 24 h after APAP injection. The results shown represent mean ± SEM of more than 5 mice per group. (*) P < 0.05, compared with APAP/PBS-treated mice.|
|HEP_23476_sm_SuppFig2.tif||3366K||Supporting Fig. 2. Evaluation of LSEC expression of CYP2E1, hepatic RBC accumulation upon APAP challenge, and Lac-induced protection against AILI in C57Bl/6 mice. (A) NPC were isolated from naïve Balb/cJ and C57Bl/6J mice. Cells isolated from 8 mice per group were pooled. LSEC were purified as CD45-negative cells by magnetic cell sorting. CYP2E1 expression was detected by immunoblot analysis, using an anti-mouse CYP2E1 (1:5000 dilution, Stressgen Biotechnologies Co.). The data shown are representative of two independent experiments producing similar results. (B) Male C57Bl/6J mice were challenged with APAP (300 mg/kg). Naïve mice (PBS-treated) were used as control. Three or five hours after APAP challenge, the livers were perfused for 1 min to remove blood within the circulation. Liver tissues were disrupted, and single cell suspensions filtered through a 100 μm cell strainer. Dead cells and hepatocytes were removed using 34% Percoll. The remaining RBC were counted using light microscopy. (*) P < 0.05 compared with naïve mice. The results shown represent mean ± SEM of 3 mice per group. (C) Male C57Bl/6J mice were i.v. injected with Lac (50 mg/kg, APAP/Lac) or PBS (APAP/PBS) at 1 and 4 h after APAP challenge (300 mg/kg). Blood was collected at 8 and 24 h after APAP injection for serum ALT measurement. (*) P < 0.05, compared with APAP/PBS-treated mice. The results shown represent mean ± SEM of 10 mice per group.|
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