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Additional Supporting Information may be found in the online version of this article.

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HEP_23476_sm_SuppFig1.tif1953KSupporting Fig. 1. Human holo-Lac and non-holo-Lac protected against AILI in mice. Male Balb/cJ mice were i.v. injected with 50 mg/kg of human holo-Lac (APAP/Holo-Lac), or non-holo-Lac (APAP/Non-holo-Lac), or PBS (APAP/PBS) at 1 and 4 h after APAP challenge (300 mg/kg). Serum ALT levels were determined at 8 and 24 h after APAP injection. The results shown represent mean ± SEM of more than 5 mice per group. (*) P < 0.05, compared with APAP/PBS-treated mice.
HEP_23476_sm_SuppFig2.tif3366KSupporting Fig. 2. Evaluation of LSEC expression of CYP2E1, hepatic RBC accumulation upon APAP challenge, and Lac-induced protection against AILI in C57Bl/6 mice. (A) NPC were isolated from naïve Balb/cJ and C57Bl/6J mice. Cells isolated from 8 mice per group were pooled. LSEC were purified as CD45-negative cells by magnetic cell sorting. CYP2E1 expression was detected by immunoblot analysis, using an anti-mouse CYP2E1 (1:5000 dilution, Stressgen Biotechnologies Co.). The data shown are representative of two independent experiments producing similar results. (B) Male C57Bl/6J mice were challenged with APAP (300 mg/kg). Naïve mice (PBS-treated) were used as control. Three or five hours after APAP challenge, the livers were perfused for 1 min to remove blood within the circulation. Liver tissues were disrupted, and single cell suspensions filtered through a 100 μm cell strainer. Dead cells and hepatocytes were removed using 34% Percoll. The remaining RBC were counted using light microscopy. (*) P < 0.05 compared with naïve mice. The results shown represent mean ± SEM of 3 mice per group. (C) Male C57Bl/6J mice were i.v. injected with Lac (50 mg/kg, APAP/Lac) or PBS (APAP/PBS) at 1 and 4 h after APAP challenge (300 mg/kg). Blood was collected at 8 and 24 h after APAP injection for serum ALT measurement. (*) P < 0.05, compared with APAP/PBS-treated mice. The results shown represent mean ± SEM of 10 mice per group.

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