Potential conflict of interest: Nothing to report.
Generation of endoderm-derived human induced pluripotent stem cells from primary hepatocytes†
Article first published online: 1 MAR 2010
Copyright © 2010 American Association for the Study of Liver Diseases
Volume 51, Issue 5, pages 1810–1819, May 2010
How to Cite
Liu, H., Ye, Z., Kim, Y., Sharkis, S. and Jang, Y.-Y. (2010), Generation of endoderm-derived human induced pluripotent stem cells from primary hepatocytes. Hepatology, 51: 1810–1819. doi: 10.1002/hep.23626
- Issue published online: 22 APR 2010
- Article first published online: 1 MAR 2010
- Accepted manuscript online: 1 MAR 2010 12:00AM EST
- Manuscript Accepted: 16 FEB 2010
- Manuscript Received: 23 NOV 2009
- National Institutes of Health grant. Grant Number: DK O70971
- Maryland Stem Cell Research Fund grants. Grant Numbers: 103655, 103974
Additional Supporting Information may be found in the online version of this article.
|HEP_23626_sm_suppfig-1.tif||14524K||Supporting Figure 1 (a). Representative morphologies of hHiPS10 growing on MEF (left panel) and Matrigel (right panel, ×100) Scale bars: 100 μm. (b). Fingerprinting of iPS cells. DNA fingerprinting analysis showed that the hepatocyte-derived iPS cell lines are originated from primary hepatocytes (PH) and have a distinct signature from 293T cells and human ES (H1) cells. (c). Karyotyping of hHiPS cells. After 40 passages, these hHiPS cells (including hHiPS11 line shown here) showed normal karyotypes.|
|HEP_23626_sm_suppfig-2.tif||11006K||Supporting Figure 2. Representative immunofluorescence analysis of hHiPS cell lines (hHiPS6, 11, and 14, a-c respectively). Clear expression of the ES cell surface antigens SSEA4 and TRA-1-60, the nuclear transcription factors OCT4 and NANOG are observed (×200).|
|HEP_23626_sm_suppfig-3.tif||991K||Supporting Figure 3. (a) A diagram of human MSC derived iPS generation protocol. (b) Representative immunofluorescence analysis of human MSC derived iPS cells. Clear expression of the ES cell surface antigens SSEA4 and TRA-1-60, the nuclear transcription factors OCT4 and NANOG are observed (×200).|
|HEP_23626_sm_suppfig-4.tif||92K||Supporting Figure 4. AFP expression of primary human hepatocytes before iPS reprogramming.|
|HEP_23626_sm_suppfig-5.tif||3446K||Supporting Figure 5. Differentiation of iMSC cells into hepatic cells. The FACS and immunofluorescence analysis showed that 80 to 90% of the induced iMSC cells expressed each stage hepatic markers. (a) iMSC derived CXCR4+ DE cells at 5 days post-initial Activin A treatment. (b) Immunofluorescence analysis of the expression of AFP (HP marker) in differentiating iMSC cells at 10 days post-initial Activin A treatment ×100. Scale bars, 100 μm. The iMSC derived mature hepatocytes (c) possessed proteins indicative of functional hepatocytes, including ALB (green), AAT (red) at day 20 after differentiation initiation. ×200, Scale bars, 100 μm.|
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