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Additional Supporting Information may be found in the online version of this article.

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HEP_23739_sm_SuppFig-1.tif1573KSupplementary Figure 1 A. Mouse LEC and macrophage (positive control) were immunostained with F4/80 and quantified. B. Mouse LEC and macrophage (positive control) were immunostained for CD11b and quantified. C. Mouse LEC and hepatic stellate cells (positive control) were immunostained for PDGFRβ and quantified.
HEP_23739_sm_SuppFig-2.tif2209KSupplementary Figure 2 A. Quantification of LEC viability after transfection of TLR4 siRNA using Calcein, AM. B. Calcein, AM is hydrolysed to Calcein by living cells (green) and bright field micrograph of the live cells is shown (left panel). C. Quantification of LEC viability after transfection MyD88 siRNA using Calcein. D. Representative pictures showing the viability of MyD88 silenced LEC (green).
HEP_23739_sm_SuppFig-3.tif1097KSupplementary Figure 3 A. Human LEC were transfected with TRAM specific siRNA or control siRNA and tubulogenesis was measured (N=3; mean ± SEM). Western blot insert is shown depicting effective TRAM knockdown with siRNA.
HEP_23739_sm_SuppFig-4.tif965KSupplementary Figure 4 Primary LEC were isolated from TLR4-WT and TLR4-MT mice were plated and cell proliferation rate was measured using MTS assay (N-3, mean ±SEM).
HEP_23739_sm_SuppFig-5.tif2104KSupplementary Figure 5 A. Differences in gene expression between TLR4-WT and TLR4-MT LEC. B. Micrographs of representative gelatin insitu zymography of TLR4-WT and TLR4-MT mouse liver tissue is depicted (arrows; darker regions indicate the areas of degradation of fluorescent gelatin, white bar depicts the margin of liver tissue).
HEP_23739_sm_SuppFig-6.tif2713KSupplementary Figure 6 A. Quantification of Aquaporin-1 (AQP-1) staining in sham and BDL induced TLR4-WT and TLR4-MT mice using Metamorph software is depicted (N=4; mean ± SEM, * indicates P<0.04, TLR4-WT BDL vs TLR4-MT BDL). B. Representative micrographs from each group are depicted. C. Quantification of AQP-1 staining in CCl4 induced TLR4-WT and TLR4-MT mice is depicted (N=6; mean ± SEM, * indicates P<0.04, TLR4-WT CCl4 vs TLR4-MT CCl4). D. Representative micrographs from each group are depicted; inset depicts higher magnification images from the same field.

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