p130Cas, Crk-associated substrate plays essential roles in liver development by regulating sinusoidal endothelial cell fenestration

Authors

  • Tatsuya Tazaki,

    1. Departments of Disease Model, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan
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    • These authors contributed equally to this work.

  • Takaaki Sasaki,

    1. Departments of Disease Model, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan
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    • These authors contributed equally to this work.

  • Kenta Uto,

    1. Department of Pathology, Tokyo Women's Medical University, Tokyo, Japan
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  • Norimasa Yamasaki,

    1. Departments of Disease Model, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan
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  • Satoshi Tashiro,

    1. Cellular Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan
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  • Ryuichi Sakai,

    1. Growth Factor Division, National Cancer Center Research Institute, Tokyo, Japan
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  • Minoru Tanaka,

    1. Promotion of Independence for Young Investigators, Institute of Molecular and Cellular Biosciences, Faculty of Medicine and Graduate School of Medicine, University of Tokyo, Tokyo, Japan
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  • Hideaki Oda,

    1. Department of Pathology, Tokyo Women's Medical University, Tokyo, Japan
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  • Zen-Ichiro Honda,

    Corresponding author
    1. Department of Allergy and Rheumatology, Faculty of Medicine and Graduate School of Medicine, University of Tokyo, Tokyo, Japan
    2. Department of Health Service for Hospital Employees, Tokyo University Hospital, Tokyo, Japan
    • Department of Allergy and Rheumatology, Faculty of Medicine and Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-Ku, Tokyo 113-8655, Japan
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  • Hiroaki Honda

    Corresponding author
    1. Departments of Disease Model, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan
    • Department of Disease Model, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-Ku, Hiroshima 734-8553, Japan
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  • Potential conflict of interest: Nothing to report.

Abstract

p130Cas, Crk-associated substrate (Cas), is an adaptor/scaffold protein that plays a central role in actin cytoskeletal reorganization. We previously showed that mice in which Cas was deleted (Cas−/−) died in utero because of early cardiovascular maldevelopment. To further investigate the in vivo roles of Cas, we generated mice with a hypomorphic Cas allele lacking the exon 2–derived region (CasΔex2ex2), which encodes Src homology domain 3 (SH3) of Cas. CasΔex2ex2 mice again died as embryos, but they particularly showed progressive liver degeneration with hepatocyte apoptosis. Because Cas expression in the liver is preferentially detected in sinusoidal endothelial cells (SECs), the observed hepatocyte apoptosis was most likely ascribable to impaired function of SECs. To address this possibility, we stably introduced a Cas mutant lacking the SH3 domain (Cas ΔSH3) into an SEC line (NP31). Intriguingly, the introduction of Cas ΔSH3 induced a loss of fenestrae, the characteristic cell-penetrating pores in SECs that serve as a critical route for supplying oxygen and nutrients to hepatocytes. The disappearance of fenestrae in Cas ΔSH3–expressing cells was associated with an attenuation of actin stress fiber formation, a marked reduction in tyrosine phosphorylation of Cas, and defective binding of Cas to CrkII. Conclusion: Cas plays pivotal roles in liver development through the reorganization of the actin cytoskeleton and formation of fenestrae in SECs. HEPATOLOGY 2010

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