These authors contributed equally to this study.
A novel role for thyroid-stimulating hormone: Up-regulation of hepatic 3-hydroxy-3-methyl-glutaryl-coenzyme a reductase expression through the cyclic adenosine monophosphate/protein kinase A/cyclic adenosine monophosphate–responsive element binding protein pathway†
Article first published online: 11 JUN 2010
Copyright © 2010 American Association for the Study of Liver Diseases
Volume 52, Issue 4, pages 1401–1409, October 2010
How to Cite
Tian, L., Song, Y., Xing, M., Zhang, W., Ning, G., Li, X., Yu, C., Qin, C., Liu, J., Tian, X., Sun, X., Fu, R., Zhang, L., Zhang, X., Lu, Y., Zou, J., Wang, L., Guan, Q., Gao, L. and Zhao, J. (2010), A novel role for thyroid-stimulating hormone: Up-regulation of hepatic 3-hydroxy-3-methyl-glutaryl-coenzyme a reductase expression through the cyclic adenosine monophosphate/protein kinase A/cyclic adenosine monophosphate–responsive element binding protein pathway. Hepatology, 52: 1401–1409. doi: 10.1002/hep.23800
Potential conflict of interest: Nothing to report.
- Issue published online: 11 JUN 2010
- Article first published online: 11 JUN 2010
- Manuscript Accepted: 2 JUN 2010
- Manuscript Received: 25 MAR 2010
- Supported by the Natural Science Foundation of China (30971409) and the Natural Science Foundation of Shandong Province ZR2009CZ009
Additional Supporting Information may be found in the online version of this article.
|HEP_23800_sm_suppfig1.tif||60K||Supporting Figure 1. The effects of TSH on HMGCR protein in human primary hepatocytes (HPH) and mouse liver cell line BNL.|
|HEP_23800_sm_suppfig2.tif||793K||Supporting Figure 2. Infection efficiency of lentivirus in L-02 cells. The cells were treated with TSHR-specific lentivirus-mediated RNA interference (TSHR-RNAi lentivirus) or with non-targeting RNAi as a negative control (NS lentivirus). Infection efficiency was observed by fluorescent microscopy: b, bright field photos; g, green fluorescence photos (original magnification ×200).|
|HEP_23800_sm_suppfig3.tif||191K||Supporting Figure 3. RNAi in BNL cells. (A) The knockdown efficiency of TSHR by 1# or 2# siRNA was detected using qPCR. Two different siRNA (1# and 2#) strongly inhibited TSHR expression. **P< 0.01 versus control. (B) siRNA effectively suppressed TSH-stimulated HMGCR protein expression (upper).Treatment of cells with the transfection reagent (TR), non-targeting siRNA (NS siRNA), 1# siRNA alone or 2# siRNA alone had no effect on HMGCR expression (lower).|
|HEP_23800_sm_suppfig4.tif||705K||Supporting Figure 4. The immunofluorescence images of TSHR in human hepatocytes. The blue colour depicts the DAPI stained nucleus (A1, B1 and C1). The green colour represents TSHR protein (A2 and B2, FITC-conjugated), The merged images of TSHR and DAPI were obtained after superposition of the green and blue channels (A3 and B3). TSHR is localized on the membranes of human hepatocytes (B2). As the positive controls (A1-A3), human thyrocytes expressed TSHR. The negative controls (C1-C3) of human hepatocytes were performed, as described, without the primary antibody and were replaced by IgG (original magnification × 400).|
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