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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
HEP_23800_sm_suppfig1.tif60KSupporting Figure 1. The effects of TSH on HMGCR protein in human primary hepatocytes (HPH) and mouse liver cell line BNL.
HEP_23800_sm_suppfig2.tif793KSupporting Figure 2. Infection efficiency of lentivirus in L-02 cells. The cells were treated with TSHR-specific lentivirus-mediated RNA interference (TSHR-RNAi lentivirus) or with non-targeting RNAi as a negative control (NS lentivirus). Infection efficiency was observed by fluorescent microscopy: b, bright field photos; g, green fluorescence photos (original magnification ×200).
HEP_23800_sm_suppfig3.tif191KSupporting Figure 3. RNAi in BNL cells. (A) The knockdown efficiency of TSHR by 1# or 2# siRNA was detected using qPCR. Two different siRNA (1# and 2#) strongly inhibited TSHR expression. **P< 0.01 versus control. (B) siRNA effectively suppressed TSH-stimulated HMGCR protein expression (upper).Treatment of cells with the transfection reagent (TR), non-targeting siRNA (NS siRNA), 1# siRNA alone or 2# siRNA alone had no effect on HMGCR expression (lower).
HEP_23800_sm_suppfig4.tif705KSupporting Figure 4. The immunofluorescence images of TSHR in human hepatocytes. The blue colour depicts the DAPI stained nucleus (A1, B1 and C1). The green colour represents TSHR protein (A2 and B2, FITC-conjugated), The merged images of TSHR and DAPI were obtained after superposition of the green and blue channels (A3 and B3). TSHR is localized on the membranes of human hepatocytes (B2). As the positive controls (A1-A3), human thyrocytes expressed TSHR. The negative controls (C1-C3) of human hepatocytes were performed, as described, without the primary antibody and were replaced by IgG (original magnification × 400).
HEP_23800_sm_suppinfo.doc105KSupporting Information
HEP_23800_sm_supptab.doc55KSupporting Table

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