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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
HEP_23804_sm_SuppFig1.tif3275KSupporting Information Figure 1. ROS expression in different liver cell types after chronic CCl4 treatment: Liver fibrosis was induced by 16 injections (every 3 days) of CCl4. (A-C) Double immunofluorescence staining for 4-hydroxynonenal (4-HNE) (red fluorescence, upper panel) and (A) F4/80 (KC), (B) αSMA (HSC) or (C) Pan-cytokeratin (HEP) (all green fluorescence, middle panel). The liver sections were from CCl4-treated mice. Arrow head indicates double positive cells. v, vessel lumens. Original magnification, x200. Magnification of the inset, x400.
HEP_23804_sm_SuppFig2.tif3563KSupporting Information Figure 2. ROS generation in Kupffer cells and stellate cells in WT and NOX-deficient (p47phox KO) mice fed an MCD diet: (A-D) Double immunofluorescence staining for 4-hydroxynonenal (4-HNE) (red fluorescence, upper panel) and (A, C) F4/80 (KC) or (B, D) αSMA (HSC) (all green fluorescence, middle panel). The liver sections were from (A-D) WT and NOX-deficient mice fed an MCD diet for 10 weeks. Closed and open arrow heads indicate double positive cells and ROS negative cells, respectively. v, vessel lumens. Original magnification, x200. Magnification of the inset, x400.

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