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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
HEP_23921_sm_SuppInfoFigure1A-B.tif57KSupporting Figure 1. Effects of biliverdin (BV) and bilirubin (BR) on proliferation and toxicity in cultures of Huh5-15NS (A, B & E) and Huh7.5FL replicon cells (C, D & F). Replicon cells were seeded into culture wells (1 × 104 cells/well,) and incubated with BV at the indicated concentrations (micromolar) in culture medium containing 5 or 10% FBS. Replicate culture wells (5 per group) were assayed daily using MTT assay (A-D) or cell counting after trypan dye exclusion (E-F). In another experiment, (G-H) parallel groups of either replicon line (5 wells per group) were incubated with either biliverdin or bilirubin at the indicated concentrations. Forty-eight hours after plating MTT absorbance was determined as described in the methods. Each point represents the mean of 5 determinations. Standard error bars have been omitted in figs A-F for clarity, but are included for figs G-H. By ANOVA, there were no significant differences between treatment groups.
HEP_23921_sm_SuppInfoFigure1C-D.tif57KSupporting Figure 1. Effects of biliverdin (BV) and bilirubin (BR) on proliferation and toxicity in cultures of Huh5-15NS (A, B & E) and Huh7.5FL replicon cells (C, D & F). Replicon cells were seeded into culture wells (1 × 104 cells/well,) and incubated with BV at the indicated concentrations (micromolar) in culture medium containing 5 or 10% FBS. Replicate culture wells (5 per group) were assayed daily using MTT assay (A-D) or cell counting after trypan dye exclusion (E-F). In another experiment, (G-H) parallel groups of either replicon line (5 wells per group) were incubated with either biliverdin or bilirubin at the indicated concentrations. Forty-eight hours after plating MTT absorbance was determined as described in the methods. Each point represents the mean of 5 determinations. Standard error bars have been omitted in figs A-F for clarity, but are included for figs G-H. By ANOVA, there were no significant differences between treatment groups.
HEP_23921_sm_SuppInfoFigure1E-F.tif57KSupporting Figure 1. Effects of biliverdin (BV) and bilirubin (BR) on proliferation and toxicity in cultures of Huh5-15NS (A, B & E) and Huh7.5FL replicon cells (C, D & F). Replicon cells were seeded into culture wells (1 × 104 cells/well,) and incubated with BV at the indicated concentrations (micromolar) in culture medium containing 5 or 10% FBS. Replicate culture wells (5 per group) were assayed daily using MTT assay (A-D) or cell counting after trypan dye exclusion (E-F). In another experiment, (G-H) parallel groups of either replicon line (5 wells per group) were incubated with either biliverdin or bilirubin at the indicated concentrations. Forty-eight hours after plating MTT absorbance was determined as described in the methods. Each point represents the mean of 5 determinations. Standard error bars have been omitted in figs A-F for clarity, but are included for figs G-H. By ANOVA, there were no significant differences between treatment groups.
HEP_23921_sm_SuppInfoFigure1G-H.tif64KSupporting Figure 1. Effects of biliverdin (BV) and bilirubin (BR) on proliferation and toxicity in cultures of Huh5-15NS (A, B & E) and Huh7.5FL replicon cells (C, D & F). Replicon cells were seeded into culture wells (1 × 104 cells/well,) and incubated with BV at the indicated concentrations (micromolar) in culture medium containing 5 or 10% FBS. Replicate culture wells (5 per group) were assayed daily using MTT assay (A-D) or cell counting after trypan dye exclusion (E-F). In another experiment, (G-H) parallel groups of either replicon line (5 wells per group) were incubated with either biliverdin or bilirubin at the indicated concentrations. Forty-eight hours after plating MTT absorbance was determined as described in the methods. Each point represents the mean of 5 determinations. Standard error bars have been omitted in figs A-F for clarity, but are included for figs G-H. By ANOVA, there were no significant differences between treatment groups.
HEP_23921_sm_suppinfo.doc71KSupporting Information Methods

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