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HEP_24037_sm_suppfigure1.tif42080KLiver pERK levels - histology. Immunohistochemical staining for phosphorylated ERK (pERK). Examples of immunohistochemical staining for the 0h (hepatectomy) and 72h time point are shown, illustrating the corresponding time points in figure 4. The left panels show nuclear pERK staining at the time of hepatectomy, the right panels are representative of pERK staining at the 72h time point. The top row illustrates the findings in a vehicle treated animal, the findings in the sorafenib treatment groups are shown in the lower rows.
HEP_24037_sm_suppfigure2.tif361KHGF hepatic levels. HGF protein content measured by ELISA from whole liver lysate, showing protein levels at baseline (hepatectomy) and at sacrifice. A, animals treated with sorafenib or vehicle until one day before surgery. B, animals treated with sorafenib or vehicle continuously. C, animals treated with sorafenib or vehicle after surgery. Black bars: vehicle treated mice; grey bars: sorafenib treated mice. At the time of hepatectomy there was a slight increase in HGF levels in the animals that stopped sorafenib one day before hepatectomy when compared to controls. This was also observed at 72h after hepatectomy, however, neither increase were significative (A). The animals receiving continuous sorafenib treatment presented decreased HGF levels at 24h when compared to controls, and also when compared to baseline levels (0h) (B). The group receiving continuous treatment and the group starting sorafenib after hepatectomy both showed elevated HGF levels at 120h when compared to controls, although this was not significant (B and C). Values are mean ± SD. * indicates statistical significance (p<0.05) versus vehicle treated control mice.

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